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. 2020 Jul 24:2020:7035847.
doi: 10.1155/2020/7035847. eCollection 2020.

Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

Shengchao Xu  1 , Chi Hu  2 , Zhiqiang Xiao  3 , Chengke Luo  1 , Zhixiong Liu  1
Affiliations

Downregulation of CyclophilinA/CD147 Axis Induces Cell Apoptosis and Inhibits Glioma Aggressiveness

Shengchao Xu et al. Biomed Res Int. .

Abstract

Gliomas are the most common primary tumors in the brain with poor prognosis. Previous studies have detected high expression of Cyclophilin A (CyPA) and CD147, respectively, in glioma. However, the correlation between their expressions and glioma prognosis remains unclear. Here, we investigated the expression of CyPA and CD147 in different types of glioma and characterized their relationships with clinical features, prognosis, and cell proliferation. Results showed that CyPA and CD147 expressions were elevated in higher grade gliomas. Moreover, the knockdown of CyPA and CD147 by RNA interference significantly induced cell express apoptosis biomarkers such as Annexin V and inhibited proliferation biomarkers like EdU in glioma cells. In summary, our findings revealed that high expression of CyPA and CD147 correlated with glioma grades. Moreover, downregulation of the Cyclophilin A/CD147 axis induces cell apoptosis and inhibits glioma aggressiveness. Those indicating CyPA and CD147 could be used as both potential predictive biomarkers and a potential therapeutic target.

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Conflict of interest statement

All authors declare that there are no competing interests.

Figures

Figure 1
Figure 1
Expressions of CyPA and CD147 are associated with glioma grade, histological type, and prognosis. The expression of CyPA (a) and CD147 (b) in GBM and LGG compared with normal tissue and correlation with prognosis in TCGA and CGGA databases. The expression of CyPA (c) and CD147 (d) in different WHO grades and histological types of glioma in TCGA and CGGA database. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001, ns stands for no significance.
Figure 2
Figure 2
Expression and knockdown of CyPA and CD147 in glioma cell lines. The expressions of CyPA (a) and CD147 (b) in glioma cell lines are detected by RT-qPCR and western blot.(c) Specific si-RNA could inhibit CyPA and CD147 expression in U251 cell line. ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.
Figure 3
Figure 3
Knockdown of CyPA and CD147 reduces glioma proliferation. (a). EdU assay shows that inhibition of CyPA and CD147 significantly decreases glioma cell proliferation. U251 wild type cell is taken as the control group. (b). Flow cytometry analysis reveals the effect of CyPA and CD147 inhibition on glioma cell proliferation.
Figure 4
Figure 4
Predictive value of CyPA and CD147 in different grades of glioma. CyPA and CD147 expression pattern in different grades of glioma in TCGA database (a) and CGGA database (b) analyzed by ROC curve analysis.
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