Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Dec;117(12):3938-3951.
doi: 10.1002/bit.27533. Epub 2020 Aug 20.

Anatomical templates for tissue (re)generation and beyond

Isabella Mavaro  1   2 , Elena De Felice  3 , Antonio Palladino  1 , Livia D'Angelo  1   4 , Paolo de Girolamo  1 , Chiara Attanasio  1   2   5
Affiliations
Review

Anatomical templates for tissue (re)generation and beyond

Isabella Mavaro et al. Biotechnol Bioeng. 2020 Dec.

Abstract

Induced pluripotent stem cells (iPSCs) represent a valuable alternative to stem cells in regenerative medicine overcoming their ethical limitations, like embryo disruption. Takahashi and Yamanaka in 2006 reprogrammed, for the first time, mouse fibroblasts into iPSCs through the retroviral delivery of four reprogramming factors: Oct3/4, Sox2, c-Myc, and Klf4. Since then, several studies started reporting the derivation of iPSC lines from animals other than rodents for translational and veterinary medicine. Here, we review the potential of using these cells for further intriguing applications, such as "cellular agriculture." iPSCs, indeed, can be a source of in vitro, skeletal muscle tissue, namely "cultured meat," a product that improves animal welfare and encourages the consumption of healthier meat along with environmental preservation. Also, we report the potential of using iPSCs, obtained from endangered species, for therapeutic treatments for captive animals and for assisted reproductive technologies as well. This review offers a unique opportunity to explore the whole spectrum of iPSC applications from regenerative translational and veterinary medicine to the production of artificial meat and the preservation of currently endangered species.

Keywords: cell reprogramming; clinical applications; meat culture; species preservation; veterinary science.

PubMed Disclaimer

References

REFERENCES

    1. Armstrong, A. J., Marengo, M. S., Oltean, S., Kemeny, G., Bitting, R. L., Turnbull, J. D., & Garcia-Blanco, M. A. (2011). Circulating tumor cells from patients with advanced prostate and breast cancer display both epithelial and mesenchymal markers. Molecular Cancer Research, 9, 997-1007. https://doi.org/10.1158/1541-7786.MCR-10-0490
    1. Attanasio, C., Latancia, M. T., Otterbein, L. E., & Netti, P. A. (2016). Update on renal replacement therapy: Implantable artificial devices and bioengineered organs. Tissue Engineering-Part B: Reviews, 22, 330-340. https://doi.org/10.1089/ten.teb.2015.0467
    1. Attanasio, C., & Netti, P. A. (2017). Bioreactors for cell culture systems and organ bioengineering, In G. Orlando, G. Remuzzi, & D. F. Williams (Eds.), Kidney transplantation, bioengineering, and regeneration: Kidney transplantation in the regenerative medicine era. (1st ed., pp. 889-899). San Diego, CA: Academic Press. https://doi.org/10.1016/B978-0-12-801734-0.00064-3
    1. Baird, A. E. G., Barsby, T., & Guest, D. J. (2015). Derivation of canine induced pluripotent stem cells. Reproduction in Domestic Animals, 50, 669-676. https://doi.org/10.1111/rda.12562
    1. Ben-Arye, T., Shandalov, Y., Ben-Shaul, S., Landau, S., Zagury, Y., Ianovici, I., & Levenberg, S. (2020). Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat. Nature Food, 1, 210-220. https://doi.org/10.1038/s43016-020-0046-5

LinkOut - more resources