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. 2020 Aug 11:9:e55133.
doi: 10.7554/eLife.55133.

Imaging methods are vastly underreported in biomedical research

Affiliations

Imaging methods are vastly underreported in biomedical research

Guillermo Marqués et al. Elife. .

Abstract

A variety of microscopy techniques are used by researchers in the life and biomedical sciences. As these techniques become more powerful and more complex, it is vital that scientific articles containing images obtained with advanced microscopes include full details about how each image was obtained. To explore the reporting of such details we examined 240 original research articles published in eight journals. We found that the quality of reporting was poor, with some articles containing no information about how images were obtained, and many articles lacking important basic details. Efforts by researchers, funding agencies, journals, equipment manufacturers and staff at shared imaging facilities are required to improve the reporting of experiments that rely on microscopy techniques.

Keywords: cell biology; developmental biology; imaging; microscopy; none; reporting standards; reproducibility; science forum.

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Conflict of interest statement

GM, TP, MS No competing interests declared

References

    1. Allan C, Burel JM, Moore J, Blackburn C, Linkert M, Loynton S, Macdonald D, Moore WJ, Neves C, Patterson A, Porter M, Tarkowska A, Loranger B, Avondo J, Lagerstedt I, Lianas L, Leo S, Hands K, Hay RT, Patwardhan A, Best C, Kleywegt GJ, Zanetti G, Swedlow JR. OMERO: Flexible, model-driven data management for experimental biology. Nature Methods. 2012;9:245–253. doi: 10.1038/nmeth.1896. - DOI - PMC - PubMed
    1. Baker M. 1,500 scientists lift the lid on reproducibility. Nature. 2016;533:452–454. doi: 10.1038/533452a. - DOI - PubMed
    1. Bauchner H, Fontanarosa PB, Flanagin A, Thornton J. Scientific misconduct and medical journals. JAMA. 2018;320:1985–1987. doi: 10.1001/jama.2018.14350. - DOI - PubMed
    1. Begley CG, Ellis LM. Drug development: Raise standards for preclinical cancer research. Nature. 2012;483:531–533. doi: 10.1038/483531a. - DOI - PubMed
    1. Benjamin DJ, Berger JO, Johannesson M, Nosek BA, Wagenmakers EJ, Berk R, Bollen KA, Brembs B, Brown L, Camerer C, Cesarini D, Chambers CD, Clyde M, Cook TD, De Boeck P, Dienes Z, Dreber A, Easwaran K, Efferson C, Fehr E, Fidler F, Field AP, Forster M, George EI, Gonzalez R, Goodman S, Green E, Green DP, Greenwald AG, Hadfield JD, Hedges LV, Held L, Hua Ho T, Hoijtink H, Hruschka DJ, Imai K, Imbens G, Ioannidis JPA, Jeon M, Jones JH, Kirchler M, Laibson D, List J, Little R, Lupia A, Machery E, Maxwell SE, McCarthy M, Moore DA, Morgan SL, Munafó M, Nakagawa S, Nyhan B, Parker TH, Pericchi L, Perugini M, Rouder J, Rousseau J, Savalei V, Schönbrodt FD, Sellke T, Sinclair B, Tingley D, Van Zandt T, Vazire S, Watts DJ, Winship C, Wolpert RL, Xie Y, Young C, Zinman J, Johnson VE. Redefine statistical significance. Nature Human Behaviour. 2018;2:6–10. doi: 10.1038/s41562-017-0189-z. - DOI - PubMed

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