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. 2020 Aug 7;9(8):1004.
doi: 10.3390/plants9081004.

The Selection of Gamma-Ray Irradiated Higher Yield Rice Mutants by Directed Evolution Method

Hiroshi Kato  1 , Feng Li  2 , Akemi Shimizu  2
Affiliations

The Selection of Gamma-Ray Irradiated Higher Yield Rice Mutants by Directed Evolution Method

Hiroshi Kato et al. Plants (Basel). .

Abstract

We have succeeded in selecting four higher yield mutants from five gamma-ray irradiated high-yielding Japanese rice varieties using a novel approach. A total of 464 M2 plants which had heavier total panicle weights per plant were first selected from 9801 irradiated M2 plants. Their higher yields were confirmed by yield trials conducted for three years with a six to ten-pairwise replicated plot design. FukuhibikiH6 and FukuhibikiH8 were selected from an irradiated high-yielding variety Fukuhibiki and showed 1.2% to 22.5% higher yield than their original significantly. YamadawaraH3 was selected from an irradiated high-yielding variety Yamadawara and its yield advantages were 2.7% to 3.9%. However, there was no difference in the genotypes of the 96 SNP (single nucleotide polymorphism) markers between the higher yield mutants and their respective original varieties. The differences in the measured phenotypical traits between each mutant and its original variety were not constant and the actual differences were marginal. Therefore, the higher yields of the selected mutants were likely to have been caused by physiological traits rather than phenotypical traits. The selection method used in this study is an application of the directed evolution method which has long been commonly used in the substantial improvements of microorganisms and their proteins.

Keywords: directed evolution; gamma-ray irradiation; higher yield; mutant; physiological trait; rice.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
M4 OonariH1 and OonariH2 at maximum tillering stage.
Figure 2
Figure 2
SNP genotyping of FukuhibikiH6, YamadawaraH3, and MochidawaraH1 by 96 SNP markers in 2017.
Figure 3
Figure 3
Multi-replicated yield trial plots of MochidawaraH1, FukuhibikiH6, and FukuhibikiH8 at maximum tillering stage in paddy fields in Ibaraki, Japan, in 2018.
Figure 4
Figure 4
Yield evaluation (left) and thermo-camera observation (right) of FukuhibikiH6 and FukuhibikiH8 at maximum tillering stage in paddy field in Ibaraki, Japan, in 2019. Note: thermo-camera was “InfReC R300SR-S” of AVIONICS CO., LTD.
See this image and copyright information in PMC

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