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. 2020 Jul 13;10(15):7971-7980.
doi: 10.1002/ece3.6542. eCollection 2020 Aug.

Complete mitochondrial genome and assembled DNA barcoding analysis of Lutjanus fulgens (Valenciennes, 1830) and its comparison with other Lutjanus species

Gyamfua Afriyie  1 , Zhongduo Wang  1   2 , Zhongdian Dong  1 , Christian Ayisi Larbi  3 , Berchie Asiedu  4 , Yusong Guo  1
Affiliations

Complete mitochondrial genome and assembled DNA barcoding analysis of Lutjanus fulgens (Valenciennes, 1830) and its comparison with other Lutjanus species

Gyamfua Afriyie et al. Ecol Evol. .

Abstract

Lutjanus fulgens (Valenciennes, 1830) is a teleost species classified under the family Lutjanidae which is a native of the Eastern Atlantic Ocean. Though highly commercialized due to its abundance and good taste, the production output has declined in recent years. This is an indication of the need for effective management and conservation measures. However, accurate species identification will ensure strategic management and conservation measure. DNA-based species identification has proven its reliability in this regard via precise species identification. Several researchers have confirmed the accuracy of DNAbarcode as a species identification tool as well as species phylogeny analysis based on both the complete mitogenome and COI gene. Currently, nine specimens of L. fulgens were sampled from Ghana and subjected to DNA-based analysis, namely, complete mitochondrial DNAand COI gene (DNA barcoding) analyses. The mitogenomic result revealed that L. fulgens is made up of a 16,500 base pairs (bp) mtDNA which consists of 22 transfer RNAs, 13 protein-coding genes, and two ribosomal RNAs (GenBank Accession Number: MN398650). Furthermore, a sequence polymorphism analysis of the COIgene (MN986442-MN986450) detected two haplotypes. These haplotypes were both collected from the same fish landing site which suggests a possible cryptic linage diversity in the L. fulgens population at Vodza. According to the phylogeny examination, a close taxonomic relationship exists between L. fulgens and Lutjanus buccanella caused by a recent evolution termed as sympatric speciation. This study serves as a novel study for this species, building the foundation for future molecular-based study for this species and as a DNA barcode reference data.

Keywords: COI gene; DNA barcode; Ghana; complete mitogenome; management and conservation; phylogeny.

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Conflict of interest statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

Figures

FIGURE 1
FIGURE 1
Mitogenomic blueprint of L. fulgens
FIGURE 2
FIGURE 2
Neighbor‐joining analysis based on COI gene sequenced in this study with L. buccanella as an out‐group
FIGURE 3
FIGURE 3
Phylogenetic analysis of (a) complete mtDNA (b) COI sequence of L. fulgens with other Lutjanidae species by maximum likelihood method
See this image and copyright information in PMC

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