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. 2020 Aug 15;202(4):610-612.
doi: 10.1164/rccm.201909-1840LE.

Measurement of Short-Chain Fatty Acids in Respiratory Samples: Keep Your Assay above the Water Line

Min Yue  1 , Jae Hyun Kim  1 , Charles R Evans  1 , Maureen Kachman  1 , John R Erb-Downward  1 , Jennifer D'Souza  1 , Betsy Foxman  1 , Sara D Adar  1 , Jeffrey L Curtis  1   2 , Kathleen A Stringer  1
Affiliations

Measurement of Short-Chain Fatty Acids in Respiratory Samples: Keep Your Assay above the Water Line

Min Yue et al. Am J Respir Crit Care Med. .
No abstract available

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Figures

Figure 1.
Figure 1.
Concentrations of short-chain fatty acids detected by gas chromatography–mass spectroscopy from negative control exhaled breath condensate (EBC) and BAL samples. This includes three water samples from three separate sources. H2O A: laboratory 1 Millipore Milli-Q academic deionizer, with a nominal resistance reading of 18.2 MΩ at 25°C that uses a Q gard 2 purification pack and a Quantum EX ultrapure cartridge with a 0.22-μm membrane filter; H2O B: a newly opened bottle of high-performance liquid chromatography (HPLC)-grade water (Fisher W5–4 HPLC grade, submicron 0.5 μm filtered); H2O C: laboratory 2 Milli-Q Biocel A10 deionizer with a nominal resistance reading of 18.0 MΩ at 25°C that uses a Q gard 2 purification pack and a Quantum EZ ultrapure cartridge with a 0.22-μm membrane filter; EBC blank: a sample of water wash from the exhaled breath acquisition equipment; normal saline: a sample from a newly opened bottle of normal saline from the bronchoscopy suite. The EBC and BAL data are the median (interquartile range) of 13 paired samples. Data are either from single samples (no error bars) or represent the median + interquartile range of three or more technical replicate samples. A portal vein plasma sample acquired from a postmortem swine at the termination of an unrelated experiment was used as a positive control. SCFA = short-chain fatty acid.
Figure 2.
Figure 2.
There is no association between total (sum) short-chain fatty acid concentration (exclusive of acetate) detected in BAL fluid (R2 = 0.01; P = 0.71) and exhaled breath condensate (R2 = 0.19; P = 0.13) and the microbiome (16S ribosomal RNA copies/μl DNA) in healthy control subjects. Note the log scale of the vertical axis. EBC = exhaled breath condensate; rRNA = ribosomal RNA; SCFA = short-chain fatty acid.
See this image and copyright information in PMC

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