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. 2021:2180:607-621.
doi: 10.1007/978-1-0716-0783-1_32.

Cryopreservation of Algae

Estefania Paredes  1 , Angela Ward  2 , Ian Probert  3 , Léna Gouhier  3 , Christine N Campbell  4
Affiliations

Cryopreservation of Algae

Estefania Paredes et al. Methods Mol Biol. 2021.

Abstract

Cryopreservation has been successfully used in the banking and maintenance of cultures of microorganisms, from bacteria to yeasts, since the onset of cryobiology. Biobanking of marine biological resources is crucial for development of scientific knowledge as researchers rely on guaranteed access to reliable, stable resources. Culture collections play a key role in the provision of marine biological resources as they ensure long-term ex situ storage of biological resources that are made available for public and private sector research and education. In this chapter, we provide protocols for cryopreservation of different types of algae cultures.

Keywords: Cryopreservation; Macroalgae; Microalgae.

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References

    1. Friedl T, Day JG, Preisig HR (2004) Culture collections of algae: increasing accessibility and exploring algal biodiversity, vol 79. Nova Hedwigia, Berlin, pp 1–327. ISBN 978-3-443-02008-8
    1. Canavate JP, Lubian LM (1995) The relation between cooling rates, cryoprotectant concentrations and salinities in the cryopreservation of marine microalgae. Mar Biol 125:325–334 - DOI
    1. Day JG, Brand JJ (2006) Cryopreservation methods for maintaining microalgal cultures. In: Andersen RA (ed) Algal culturing techniques. Elsevier, Amsterdam, pp 165–187
    1. Rhodes L, Smith KF, MacKenzie L, Wood SA, Ponikla K, Harwood DT, Packer M, Munday R (2006) The Cawthron Institute Culture Collection of Micro-algae: a significant national collection. New Zeal J Mar Fresh Res 50:291–316 - DOI
    1. Paredes E, Costas D, Casal A, Cortina-Burgueño A, Lubián LM (2013) Criopreservación de microalgas marinas: Nannochloropsis gaditana Rhodomonas lens, Cylindrotheca closterium, Chaetoceros gracilis, Synechoccocus sp. e Isochrysis aff. galbana clon T-ISO. In: Foro Iberoam V, Acui RM, Cardenas S, Mancera JM, Rey-Méndez M, Lodeiros C (eds) pp 263–272. ISBN: 978-84-6959072-0

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