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. 2020 May 8;29(9):1187-1194.
doi: 10.1007/s10068-020-00766-y. eCollection 2020 Sep.

Production of protein hydrolysate from Protaetia brevitarsis seulensis (Kolbe) larvae by enzyme treatment under high pressure

Tae-Eun Kim  1 , Chong-Tai Kim  2 , Hyeon-Jae Sim  3 , Hoon-Yeon Lee  3 , Seong-Oh Kim  3 , Dong-Keun Kim  3 , Mi-Na Jo  3 , So-Jin Lee  3 , Yong-Deok Jeon  4   5 , Young-Jae Song  4 , Jong-Sik Jin  4 , Yong-Jin Cho  1
Affiliations

Production of protein hydrolysate from Protaetia brevitarsis seulensis (Kolbe) larvae by enzyme treatment under high pressure

Tae-Eun Kim et al. Food Sci Biotechnol. .

Abstract

To improve the industrial use of health-functional materials based on edible insects, the objective of this study was to establish optimal conditions for improving the quality of Protaetia brevitarsis seulensis larval (PBSL) hydrolysates. PBSL was extracted using four methodologies: atmospheric pressure 50 °C-water extraction, atmospheric pressure 95 °C-water extraction, atmospheric pressure 50 °C-water enzymatic hydrolysis, and enzyme treatment under high pressure (HPE). The quality characteristics of soluble solid content, extraction yield, total protein content, protein yield, protein content with low molecular weight (LMW) (< 1kD), and the amino acid composition of hydrolysates were compared based on the different methods. All of the quality characteristics were found to be higher for HPE extracts than for the other extracts. Under optimized HPE conditions, extraction yield, protein yield, protein content with LMW, amino acid content and the content of essential amino acids increased by 3.4, 4.4 1.4 1.5, and 1.3 times respectively, compared to the other methods.

Keywords: Amino acid; Enzymatic hydrolysis; Medium–high pressure; Protaetia brevitarsis seulensis larvae; Protein hydrolysate.

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Conflict of interest statement

Conflict of interestThe authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1
Schematic diagram of the high-pressure system. (1) Vessel, (2) Oil return system, (3) Pump, (4) Solenoid valve, (5) Booster pump, (6) Solenoid value, (7) Solenoid value, (8) Pilot check, (9) Drain vlaue, (10) Safety sensor, (11) Relief value, (12) Solenoid value, (13) Level sensor, (14) Solenoid value, (15) Safety value, (16) Drain pipe
Fig. 2
Fig. 2
Total amino acid and essential amino acid content of protein hydrolysate from Protaetia brevitarsis seulensis larvae with different solid/liquid ratio (a) or enzyme concentration (b)
See this image and copyright information in PMC

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