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. 1988 Jan;19(1):33-42.
doi: 10.1016/0166-0934(88)90005-5.

Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization

M C Fung  1 , K Y Chiu, T Weber, T W Chang, N T Chang
Affiliations

Detection and purification of a recombinant human B lymphotropic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization

M C Fung et al. J Virol Methods. 1988 Jan.

Abstract

A recombinant virus Ac373-HB was detected and purified from the transfection mixture of wild type and recombinant virus in the baculovirus expression system using a combination of limiting dilution and DNA dot-blot hybridization. This method allows for a quick and convenient way of detection and purification of recombinant virus without the need to use a plaque purification step. It is generally applicable to other expression systems besides the baculovirus system described here.

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