Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Sep 25;127(8):1112-1114.
doi: 10.1161/CIRCRESAHA.120.317128. Epub 2020 Aug 17.

LDL-Cholesterol Reduction by ANGPTL3 Inhibition in Mice Is Dependent on Endothelial Lipase

Liya Wu  1 , Mangala M Soundarapandian  2 , Adam B Castoreno  2 , John S Millar  1 , Daniel J Rader  1   3
Affiliations

LDL-Cholesterol Reduction by ANGPTL3 Inhibition in Mice Is Dependent on Endothelial Lipase

Liya Wu et al. Circ Res. .
No abstract available

Keywords: angiopoietins; cholesterol; lipoproteins; metabolism; triglycerides.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
A) Study design: 8–10 week-old WT (n=10) and EL-KO (n=10) mice on chow diet were used for the study (n=5/treatment group). Fasting bleeds are marked with *. Two independent experiments of this design were performed. The mice and protocol used were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Pennsylvania. B) Hepatic LDLR protein: Primary antibodies: anti-LDLR (1:5,000, Abcam ab52818), anti-β-Actin (1:10,000 Sigma-Aldrich A5441). C) Hepatic Angptl3 expression: mRNA by qRT-PCR and plasma ANGPTL3 levels by ELISA. Data are presented as mean±SEM. Mann-Whitney test was performed to compare between treatment groups. D) Fasting plasma lipids: Measured by autoanalyzer. E) Non-HDL-C, VLDL-C, and LDL-C: Non-HDL-C = TC minus HDL-C. Pooled plasma was fractionated by FPLC and used to determine the % of individually measured non-HDL-C that was VLDL-C and LDL-C. P-values (bold) in Figure 1D and E show overall treatment effect as per repeated-measures two-way ANOVA. Significant comparisons by Sidak’s multiple comparisons test between treatment groups are shown at the indicated time points.
See this image and copyright information in PMC

References

    1. Musunuru K, Pirruccello JP, Do R, et al. Exome Sequencing, ANGPTL3 Mutations, and Familial Combined Hypolipidemia. N Engl J Med. 2010;363:2220–2227. - PMC - PubMed
    1. Gusarova V, Alexa CA, Wang Y, et al. ANGPTL3 blockade with a human monoclonal antibody reduces plasma lipids in dyslipidemic mice and monkeys. J Lipid Res. 2015;56:1308–1317. - PMC - PubMed
    1. Gaudet D, Gipe DA, Pordy R, et al. ANGPTL3 Inhibition in Homozygous Familial Hypercholesterolemia. N Engl J Med. 2017;377:296–297. - PubMed
    1. Wang Y, Gusarova V, Banfi S, Gromada J, Cohen JC, Hobbs HH. Inactivation of ANGPTL3 reduces hepatic VLDL-triglyceride secretion. J Lipid Res. 2015;56:1296–1307. - PMC - PubMed
    1. Broedl UC, Maugeais C, Millar JS, Jin W, Moore RE, Fuki IV., Marchadier D, Glick JM, Rader DJ. Endothelial lipase promotes the catabolism of ApoB-containing lipoproteins. Circ Res. 2004;94:1554–1561. - PubMed

Publication types

MeSH terms