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. 2020:2205:143-159.
doi: 10.1007/978-1-0716-0908-8_9.

Hierarchical Modular DNA Assembly Using MetClo

Da Lin  1 , Christopher A O'Callaghan  2
Affiliations

Hierarchical Modular DNA Assembly Using MetClo

Da Lin et al. Methods Mol Biol. 2020.

Abstract

DNA assembly methods are essential for multiple applications including synthetic biology. We recently developed MetClo, a method that uses a single type IIS restriction enzyme for hierarchical modular DNA assembly. This offers great flexibility in the design of the assembly experiment and simplicity of execution. Here we describe a protocol for hierarchical assembly of large DNA constructs from modular DNA parts using the MetClo vector set, a set of assembly vectors designed for the MetClo method.

Keywords: DNA assembly; MetClo; Modular assembly; Synthetic biology; Type IIS restriction enzyme.

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