Mobius Assembly

Abstract

Mobius Assembly is a versatile and user-friendly DNA Assembly method, which facilitates rapid and simple generation of DNA constructs. Mobius Assembly combines high cloning capacity and vector toolkit simplicity to streamline combinatorial assemblies. It is a two-level hierarchical modular cloning system that enables quadruple assembly augmentation. It adopts the 4 bp standard overhangs defined by Phytobricks to promote standard part sharing, and it can be made compatible with different chassis. Furthermore, Mobius Assembly reduces domestication requirements and uses chromogenic proteins to facilitate the identification of positive assemblies.

Keywords: AarI; Chromoproteins; DNA assembly; Golden Gate Assembly; Mobius Assembly; Molecular cloning; Phytobricks; Synthetic biology.