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. 1988 Jan;16(1):77-86.
doi: 10.1016/0378-1135(88)90128-9.

Adhesion of Staphylococcus aureus and Escherichia coli to bovine udder epithelial cells

J P Opdebeeck  1 , A J Frost, D O'Boyle
Affiliations

Adhesion of Staphylococcus aureus and Escherichia coli to bovine udder epithelial cells

J P Opdebeeck et al. Vet Microbiol. 1988 Jan.

Abstract

An assay for the adhesion of tritiated thymidine-labelled Staphylococcus aureus and Escherichia coli to bovine mammary ductular epithelial cell lines was developed. The relative adhesion of 15 strains of S. aureus to these cell lines was examined. Four strains did not adhere and the remaining 11 adhered at variable levels. Adhesion to different cell lines was generally similar. Adhesion to freshly collected bovine mammary epithelial cells was significantly greater than that to cells maintained in tissue culture. The system described was demonstrated to be a suitable model for studying adhesion of mastitis-causing organisms to bovine mammary epithelial cells.

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