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. 2020 Sep;26(9):2210-2213.
doi: 10.3201/eid2609.191575.

Sequence Type Changes Associated with Decreasing Macrolide-Resistant Mycoplasma pneumoniae, Japan

Miyuki Morozumi, Takeshi Tajima, Megumi Sakuma, Michi Shouji, Hidenori Meguro, Kota Saito, Satoshi Iwata, Kimiko Ubukata

Sequence Type Changes Associated with Decreasing Macrolide-Resistant Mycoplasma pneumoniae, Japan

Miyuki Morozumi et al. Emerg Infect Dis. 2020 Sep.

Abstract

We compared sequence types (STs) of Mycoplasma pneumoniae isolates from Japan during 2002-2019. ST3 and ST14 dominated during 2002-2016, and ST7 and ST33 dominated during 2018-2019. These STs were associated with a decrease in macrolide-resistant strains after an epidemic of infection with M. pneumoniae during 2011-2012.

Keywords: Japan; Mycoplasma pneumoniae; antimicrobial resistance; bacteria; macrolide resistance; multilocus sequence typing; sequence type.

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Figures

Figure 1
Figure 1
Relationships between year of isolation and STs among 279 macrolide-susceptible M. pneumoniae and 191 macrolide-resistant M. pneumoniae from children in Japan, 2002–2019. A) All strains tested; B) macrolide-susceptible strains; C) macrolide-resistant strains. Others includes ST13 (2005), ST15 (2002, 2016), ST16 (2002, 2010), ST18 (2010), ST20 (2004), ST21 (2011), ST 22 (2003, 2006, 2016), ST29 (2016), and ST30 (2016). ST, sequence type.
Figure 2
Figure 2
Relationships between CCs and STs for Mycoplasma pneumoniae isolates determined by goeBURST (http://www.phyloviz.net), Japan. Data were obtained from isolates including 470 strains from Japan during 2002–2019 and 62 strains isolated in the United Kingdom, the United States, China, and France. Blue circles indicate isolates from Japan during 2002–2016; red circles indicate isolates from Japan during 2018–2019; and gray circles indicate isolates from the United Kingdom during 1967–2010, the United States during 1944–1994, China during 2014, and France during 1981. Among all isolates, 26 STs were identified. CC, clonal complex; ST, sequence type.
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References

    1. Lee JK, Lee JH, Lee H, Ahn YM, Eun BW, Cho EY, et al. Clonal expansion of macrolide-resistant sequence type 3 Mycoplasma pneumoniae, South Korea. Emerg Infect Dis. 2018;24:1465–71. 10.3201/eid2408.180081 - DOI - PMC - PubMed
    1. Okada T, Morozumi M, Tajima T, Hasegawa M, Sakata H, Ohnari S, et al. Rapid effectiveness of minocycline or doxycycline against macrolide-resistant Mycoplasma pneumoniae infection in a 2011 outbreak among Japanese children. Clin Infect Dis. 2012;55:1642–9. 10.1093/cid/cis784 - DOI - PubMed
    1. Zhao F, Li J, Liu J, Guan X, Gong J, Liu L, et al. Antimicrobial susceptibility and molecular characteristics of Mycoplasma pneumoniae isolates across different regions of China. Antimicrob Resist Infect Control. 2019;8:143. 10.1186/s13756-019-0576-5 - DOI - PMC - PubMed
    1. Morozumi M, Nakayama E, Iwata S, Aoki Y, Hasegawa K, Kobayashi R, et al. Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J Clin Microbiol. 2006;44:1440–6. 10.1128/JCM.44.4.1440-1446.2006 - DOI - PMC - PubMed
    1. Liu Y, Ye X, Zhang H, Wu Z, Xu X. Rapid detection of Mycoplasma pneumoniae and its macrolide-resistance mutation by Cycleave PCR. Diagn Microbiol Infect Dis. 2014;78:333–7. 10.1016/j.diagmicrobio.2013.12.002 - DOI - PubMed

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