Correction to: CRISPR-Cas9 mediated targeted disruption of FAD2-2 microsomal omega-6 desaturase in soybean (Glycine max.L)

Abstract

An amendment to this paper has been published and can be accessed via the original article.

Fig. 5

Tissue specific Quantitative real time expression analysis of CRISPR-Cas9 induced FAD2–2 gene. The relative expression level of mutant FAD2–2 gene in four different tissues of soybean including root, stem, leaf and seed. The abundance of mutant FAD2–2 transcripts was found in the leaf tissue of transgenic soybean lines. The data was normalized to that of GmActin gene (NM_001289231)

Fig. 6

Agrobacterium mediated stable transformation of soybean. From A to F showed the series of different media to acquired stable transgenic soybean plants. a Sterilized soybean seeds were germinated on germination medium. b After infection with agrobacterium the seeds were planted in co-culture medium under controlled environment. c Collection of transgenic plants on a selective medium comprising 6 mg l⁻¹glufosinate where left side medium indicates sensitivity while right side showed resistance to herbicide (glufosinate). d Glufosinate resistant seedlings were incubated on shoot elongation medium after 2 weeks. e Establishment of rootswere observed after 2 weeks of incubation on rooting medium. f A full flourish plant was shifted to sterile soil in a growth chamber