Fullerene carbon dot catalytic amplification-aptamer assay platform for ultratrace As+3 utilizing SERS/RRS/Abs trifunctional Au nanoprobes

Abstract

Under microwave conditions, Au-doped carbon dots (CD_Au) were prepared using fullerene as a precursor, and characterized in details. It is found that CD_Au can strongly catalyze the reaction of HAuCl₄-fructose to generate gold nanoparticles (AuNPs). The new nanocatalytic reaction was studied by surface-enhanced Raman scattering (SERS), resonance Rayleigh scattering (RRS) and absorption (Abs) spectrometry. Based on the specific aptamer (Apt_As)-As⁺³ reaction mediated the CD_Au-HAuCl₄-fructose nanoreaction, and the products of AuNPs as SERS/RRS/Abs trifunctional indicator nanoprobes, a new trimode Apt assay strategy was developed for detection of ultratrace As⁺³. A 0.07-0.70, 0.10-0.60 and 0.20-0.70 μg L^-1 were determined by SERS, RRS and Abs, with detection limits (DL) of 0.04, 0.06, 0.10 μg L^-1 respectively. The aptamer-regulation CD_Au catalytic amplification platform can be also used to assay 1.7-13.3 nmol L^-1 Pb²⁺ and 2.0-12 μmol L^-1 Hg²⁺, with DL of 0.80 nmol L^-1 and 0.90 μmol L^-1 respectively.

Keywords: Aptamers; As(+3); Gold-doped fullerenes carbon dots; Nanocatalytic amplification; Trimode molecular spectroscopy.