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. 2020 Oct:169:104668.
doi: 10.1016/j.pestbp.2020.104668. Epub 2020 Jul 23.

Reduced fecundity and regulation of reproductive factors in flubendiamide-resistant strains of Plutella xylostella

Shi-Qing Sun  1 , Nian-Meng Wang  1 , Jing-Jing Li  1 , Ming-Hui Jin  2 , Chao-Bin Xue  3
Affiliations

Reduced fecundity and regulation of reproductive factors in flubendiamide-resistant strains of Plutella xylostella

Shi-Qing Sun et al. Pestic Biochem Physiol. 2020 Oct.

Abstract

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of flubendiamide has led to the development of resistance in field populations and reports of control failures. In this study, the lab-selected (Rf) and field-collected (Rb) flubendiamide-resistant strains of P. xylostella with LC50 resistance ratios of 1890-fold and 1251-fold, respectively, were used, as well as a lab-reared flubendiamide-susceptible strain (S). The results showed that the fecundity of the Rf and Rb-resistant strains was significantly lower than that of S strain. The contents of vitellin and transcripts of P. xylostella vitellogenin (PxVg) and P. xylostella vitellogenin receptor (PxVgR) genes in the Rf and Rb strains were significantly higher than those of S strains at 0-48 h after adult eclosion. At 96 h after eclosion, the content of vitellin in the Rf and Rb strains did not differ significantly from those of S strains, whereas transcripts of the PxVg and PxVgR genes in the Rf and Rb strains were significantly lower than that of the S strain. The content of the juvenile hormone III (JH III), β-ecdysone (20E), and the gene expression level of P. xylostella methoprene tolerant (PxMet) in the Rf and Rb strains were significantly higher than that of the S strain. The activity of trehalase was significantly higher in the Rf and Rb strains than that of the S strain in the first to the third instar larvae, whereas in the fourth instar larvae, there was no significantly difference in the three strains. At different times after adult eclosion, the differences in trehalase activity were erratic between the strains. The transcripts of P. xylostella trehalase (PxTre) gene in the Rf and Rb strains were significantly higher than that of the S strain in most developmental stages. Here, we report differences in fecundity between flubendiamide-resistant and susceptible strains of P. xylostella and discuss gene expression of several reproductive factors, which provides a possible explanation for the mechanism of fecundity reduction concurrent with flubendiamide-resistance in P. xylostella.

Keywords: Fecundity; Flubendiamide-resistance; Hormone; Trehalase; Vitellin.

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Conflict of interest statement

Declaration of competing interest The authors have declared that they have no conflict of interest.

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