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. 2020 Nov;98(3):115140.
doi: 10.1016/j.diagmicrobio.2020.115140. Epub 2020 Jul 12.

Monitoring antibody response following SARS-CoV-2 infection: diagnostic efficiency of 4 automated immunoassays

Fleur Wolff  1 , Hafid Dahma  2 , Cécile Duterme  3 , Sigi Van den Wijngaert  2 , Olivier Vandenberg  4 , Frédéric Cotton  3 , Isabel Montesinos  2
Affiliations

Monitoring antibody response following SARS-CoV-2 infection: diagnostic efficiency of 4 automated immunoassays

Fleur Wolff et al. Diagn Microbiol Infect Dis. 2020 Nov.

Abstract

Introduction: SARS-CoV-2 seroconversion is important for epidemiological studies as well as contact tracing.

Material and methods: The antibody response against SARS-CoV-2 was examined in 111 patients with a positive qRT-PCR. Seroconversion was assessed using the Elecsys from Roche, the Liaison S1/S2 IgG from Diasorin, the IgG and IgA from Euroimmun, as well as the VIDAS IgG and IgM. Specificity was estimated based on the measurement of SARS-CoV-2 antibodies in 96 residual samples collected during a non-pandemic period.

Results: The highest overall sensitivity for detecting seroconversion was obtained using the Elecsys (81.1%), the Euroimmun with a combined detection of IgG/IgA (86.5%), and the VIDAS with a simultaneous measurement of IgG/IgM (78.4%).The Elecsys and the VIDAS IgG/IgM demonstrated a specificity as well as a positive predictive value of 100%.

Conclusions: The Elecsys and the VIDAS methods with a combination of IgG/IgM measurement demonstrated a high sensitivity with no false positive results.

Keywords: SARS-CoV-2; immunoassays; seroconversion.

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Figures

Figure 1
Figure 1
Detection rates for SARS CoV2 antibodies obtained using commercial immunoassays in 6 COVID-19 patients. The threshold value for positivity is reported for each test. Values between ≥12 and 15 for the Liaison SAsRS-CoV-2 S1/S2 IgG and between ≥0.8 and 1.1 for the Euroimmun IgG/IgA are considered as borderline data. The seroconversion was achieved earlier with the Elecsys (Patient 1, 4), the Euroimmun IgA (Patient 1, 2, and 4) and the VIDAS IgM (Patient 1, 2) tests for half of the studied patients.
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