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Comparative Study
. 1988 May;170(5):2414-7.
doi: 10.1128/jb.170.5.2414-2417.1988.

Microcin B17, a novel tool for preparation of maxicells: identification of polypeptides encoded by the IncFII minireplicon pMccB17

O Mayo  1 , C Hernández-Chico, F Moreno
Affiliations
Comparative Study

Microcin B17, a novel tool for preparation of maxicells: identification of polypeptides encoded by the IncFII minireplicon pMccB17

O Mayo et al. J Bacteriol. 1988 May.

Abstract

The DNA replication inhibitor peptide microcin B17 is shown to be a useful tool for preparing Escherichia coli maxicells. To illustrate its usefulness, we have identified polypeptides synthesized from pMccB17 and R100 IncFII miniplasmids. After comparing the respective polypeptides and the miniplasmid restriction maps, we concluded that these plasmids share extensive homology in the basic replicon but are different for an adjacent region (parD) that is involved in plasmid stability and maintenance.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Proteins. 1986 Nov;1(3):230-8 - PubMed
    1. J Bacteriol. 1979 Jan;137(1):692-3 - PubMed
    1. J Bacteriol. 1979 Jun;138(3):705-14 - PubMed
    1. Mol Gen Genet. 1980;179(3):527-37 - PubMed

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