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. 1978 Dec;36(6):847-50.
doi: 10.1128/aem.36.6.847-850.1978.

Quantitative assay for algal chemotaxis

R D Sjoblad, I Chet, R Mitchell

Quantitative assay for algal chemotaxis

R D Sjoblad et al. Appl Environ Microbiol. 1978 Dec.

Abstract

A quantitative capillary assay is described for measuring chemoreception in the neritic and littoral unicellular alga Dunaliella tertiolecta. Lucite chemotaxis plates were used in the assay with 3-microliter capillaries. A Coulter Counter was employed to determine algal cell numbers. D. tertiolecta is attracted to ammonium ion with a maximum positive response at 10(-3) M. Inclusion of calcium and L-methionine in the chemotaxis medium stimulates algal chemoreception, although neither chemical is essential for motility. Attraction of the chlorophyte to ammonium is dependent on time of incubation, cell density, and pH. The optimum pH for attraction was found to be 6.25.

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References

    1. Lipids. 1968 Jan;3(1):5-13 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3312-6 - PubMed
    1. Nature. 1977 Nov 3;270(5632):66-7 - PubMed
    1. FEBS Lett. 1974 Mar 15;40(1):53-6 - PubMed
    1. Science. 1969 Dec 26;166(3913):1588-97 - PubMed

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