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. 2020 Nov 1;15(11):1809847.
doi: 10.1080/15592324.2020.1809847. Epub 2020 Aug 25.

Indeterminate domain 3 negatively regulates plant erectness and the resistance of rice to sheath blight by controlling PIN-FORMED gene expressions

Si Ting Wang  1 , Xiao Fan Guo  2 , Ting Shan Yao  3 , Yuan Hu Xuan  1
Affiliations

Indeterminate domain 3 negatively regulates plant erectness and the resistance of rice to sheath blight by controlling PIN-FORMED gene expressions

Si Ting Wang et al. Plant Signal Behav. .

Abstract

Plant architecture and disease resistance are the key factors that control the production of yield. However, the mechanism behind these factors is largely unknown. In this study, we identified that indeterminate domain 3 (IDD3) was obviously induced by inoculation of Rhizoctonia solani AG1-IA. Plants that overexpressed IDD3 (IDD3 OX) were more susceptible, while idd3 mutants showed a similar response to sheath blight disease compared with wild-type plants. Interestingly, IDD3 OX plants developed a wider tiller angle and exhibited altered shoot gravitropism, while idd3 knock-out mutants showed no visible morphological differences compared with the wild-type plants. IDD3 is ubiquitously expressed in different tissues and stages, and the IDD3 transcript was induced by exogenously applied auxin. Expression of the PIN-FORMED (PIN) and Aux/IAA genes was altered in IDD3 OX compared with wild-type plants. Furthermore, IDD3 OX plants are sensitive to auxin and the polar auxin transporter inhibitor N-1-naphthylphalamic acid (NPA). Further yeast-one hybrid, chromatin immunoprecipitation (ChIP) and transient assays revealed that IDD3 directly represses PIN1b via promoter binding. Inoculation with R. solani indicated that PIN1b RNAi plants are more susceptible to sheath blight disease (ShB) compared with the wild-type. Taken together, our analyses suggest that IDD3 controls plant architecture and the resistance of rice to ShB via the regulation of PIN auxin transporter genes.

Keywords: PIN; Indeterminate domain 3; planting density; rice; sheath blight.

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Figures

Figure 1.
Figure 1.
Patterns of expression of IDD3 in rice
Figure 2.
Figure 2.
Phenotypic expression of IDD3 mutants and overexpression plants
Figure 3.
Figure 3.
Auxin-dependent levels of IDD3 and IDD3 overexpression phenotype
Figure 4.
Figure 4.
IDD3 directly regulates the expression of PIN1b.
Figure 5.
Figure 5.
The response of PIN1b RNAi plants to sheath blight
See this image and copyright information in PMC

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