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. 2020 Aug 24;9(9):2735.
doi: 10.3390/jcm9092735.

Oral and Fecal Microbiota in Lynch Syndrome

Roberto Ferrarese  1 , Raffaella Alessia Zuppardo  2 , Marta Puzzono  2 , Alessandro Mannucci  3 , Virginia Amato  1 , Ilaria Ditonno  3 , Maria Grazia Patricelli  4 , Annalisa Russo Raucci  4 , Massimo Clementi  1   3 , Ugo Elmore  5 , Riccardo Rosati  3   5 , Pier Alberto Testoni  2   3 , Nicasio Mancini  1   3 , Giulia Martina Cavestro  2   3
Affiliations

Oral and Fecal Microbiota in Lynch Syndrome

Roberto Ferrarese et al. J Clin Med. .

Abstract

Background: The role of microbiota in Lynch syndrome (LS) is still under debate. We compared oral and fecal microbiota of LS saliva and stool samples with normal healthy controls (NHC).

Methods: Total DNA was purified from feces and saliva to amplify the V3-V4 region of the 16s rRNA gene. Sequences with a high-quality score and length >250 bp were used for taxonomic analysis with QIIME software.

Results: Compared to NHC, LS fecal samples demonstrated a statistically significant increase of Bacteroidetes and Proteobacteria and a significant decrease of Firmicutes at the phylum level and of Ruminococcaceae at the family level. Moreover, LS oral samples exhibited a statistically significant increase of Veillonellaceae and Leptotrichiaceae and a statistically significant decrease of Pasteurellaceae. A beta-diversity index allowed differentiation of the two groups.

Conclusions: A peculiar microbial signature is associated with LS, similar to that of sporadic colorectal cancer and Crohn's disease. These data suggest a possible role of proinflammatory bacteria in tumor development in a condition of genetic predisposition, such as LS.

Keywords: colorectal neoplasms; gut microbiota; lynch syndrome; oral microbiota.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Analysis of fecal samples. (a) Beta diversity analysis of fecal samples with respect to subject status: Lynch syndrome patients = PT (red); control subjects = CTRL (blue). Weighted UniFrac distance metric and principal component analysis were used to perform beta-diversity analysis. A cluster can be observed between the two groups, confirmed by Adonis analysis (p: 0.006). Statistical analysis: Adonis. Statistical significance: p < 0.05. (b,c) Taxonomic composition of stool samples from Lynch syndrome patients (PT) and control subjects (CTRL) at phylum (b) and family (c) levels. Relative bacterial abundances are expressed as average percentage. Statistical significance: p < 0.05.
Figure 2
Figure 2
Analysis of salivary samples. (a) Beta diversity analysis of salivary samples with respect to subject status: Lynch syndrome patients = PT (red); control subjects = CTRL (blue). Weighted UniFrac distance metric and principal component analysis were used to perform beta-diversity analysis. A cluster can be observed between the two groups, confirmed by Adonis analysis (p: 0.001). Statistical analysis: Adonis. Statistical significance: p < 0.05. (b,c) Taxonomic composition of oral samples from Lynch syndrome patients (PT) and control subjects (CTRL) at phylum (b) and family (c) levels. Relative bacterial abundances are expressed as average percentage. * Statistical significance: p < 0.05.
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