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Comment
. 2021 Feb;82(2):282-327.
doi: 10.1016/j.jinf.2020.08.031. Epub 2020 Aug 25.

Characterizing COVID-19 severity, epidemiology and SARS-CoV-2 genotypes in a regional business hub of China

Yan Yan  1 , Bin Liu  2 , Hui Ding  2 , Xu Wang  2 , Yaping Dai  2 , Difei Ding  2 , Hui Yu  2 , Mingchao Sha  2 , Chunyan Lui  2 , Davgadorj Chantsalmaa  2 , Yuanwang Qiu  3 , Lihua Huang  4 , Qinxue Hu  5
Affiliations
Comment

Characterizing COVID-19 severity, epidemiology and SARS-CoV-2 genotypes in a regional business hub of China

Yan Yan et al. J Infect. 2021 Feb.
No abstract available

Keywords: Covid-19; Disease severity; Epidemiology; Genotype; Sars-cov-2.

PubMed Disclaimer

Conflict of interest statement

Declaration of Competing Interest The authors declare no competing interest.

Figures

Fig. 1
Fig. 1
Fifty-five SARS-CoV-2 infected cases and migration map. A. Migration world map of SARS-CoV-2 cases to Wuxi city, Jiangsu Province, China (from January to March 2020). B. Geographic migration areas of the SARS-CoV-2 epidemic in Wuxi. Generation 1 was imported cases, and generation 2 and generation 3 cases were found in Wuxi. The number of cases is shown in brackets, while ★ and the number beside it represents the specimen number of sequencing-positive specimen. These positive samples were registered in NCBI GenBank and accession numbers obtained. C and D. Comparison of mild and severe illness case numbers and percentages (mark above the columns) through epidemic generation and sex groups. E. Composition comparison with or without comorbid conditions in severe and mild illness cases. Percentages of severe cases or cases with comorbidities are marked above the columns. F. Composition percentage of each age group is marked on the pie chart. The number of severe illnesses is marked in brackets below each age composition percentage.
Fig. 2
Fig. 2
Maximum likelihood method (MLM) analysis and haplotype analysis of SARS-CoV-2. A. Phylogenetic analysis of SARS-CoV-2. Analyses of the mutations at key sites in the genome and the phylogenetic tree of the Wuxi strains. PCR primers for SARS-CoV-2 ORF1a and ORF8 were designed referring to the reference strains downloaded from the Coronavirus Global Shared Database website (CGSD, http://nmdc.cn/coronavirus) using Primer Premier 5.0 software, and the primer sequences are as follows: 5′-AAT AAT TGG TTG AAG CAG C-3′ (ORF1a sense) and 5′-TCT ATA AGT TTT GAT GGT-3′ (ORF1a antisense), and 5′-CTT ATT ATC TTT TGG TTC TCC-3′ (ORF8 sense) and 5′-GGG GTC CAT TAT CAG ACA TTT T-3′ (ORF8 antisense), yielding 378 bp and 465 bp DNA products, respectively. Genome sequences were extracted and aligned using BioEdit 7.02 software. Phylogenetic trees for targeted genomes were constructed using Molecular Evolutionary Genetics Analysis (MEGA, version 6.06) by the maximum likelihood method (MLM) with 1000 replicates. A black solid circle (●) was plotted before Wuxi strains. The region of the country was expressed in abbreviated form. Each sequence was marked with the accession number and the collection date in the figure. Bootstrap values above 50 are shown. B. Haplotype analysis of SARS-CoV-2. Alignment analysis of nucleotide and amino acid sequences was performed with MEGA 6.06. Nucleotide and amino acid sequences of Wuxi strains and reference strains are shown as single letters or dots when they are the same.
See this image and copyright information in PMC

Comment on

  • Characteristics of COVID-19 infection in Beijing.
    Tian S, Hu N, Lou J, Chen K, Kang X, Xiang Z, Chen H, Wang D, Liu N, Liu D, Chen G, Zhang Y, Li D, Li J, Lian H, Niu S, Zhang L, Zhang J. Tian S, et al. J Infect. 2020 Apr;80(4):401-406. doi: 10.1016/j.jinf.2020.02.018. Epub 2020 Feb 27. J Infect. 2020. PMID: 32112886 Free PMC article.

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