Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2020 Nov;104(4):880-891.
doi: 10.1111/tpj.14973. Epub 2020 Sep 23.

Nanoparticle-mediated gene transformation strategies for plant genetic engineering

Zongyou Lv  1   2 , Rui Jiang  1 , Junfeng Chen  1 , Wansheng Chen  1   2
Affiliations
Free article
Review

Nanoparticle-mediated gene transformation strategies for plant genetic engineering

Zongyou Lv et al. Plant J. 2020 Nov.
Free article

Abstract

Plant genetic engineering, a recent technological advancement in the field of plant science, is an important tool used to improve crop quality and yield, to enhance secondary metabolite content in medicinal plants or to develop crops for sustainable agriculture. A new approach based on nanoparticle-mediated gene transformation can overcome the obstacle of the plant cell wall and accurately transfer DNA or RNA into plants to produce transient or stable transformation. In this review, several nanoparticle-based approaches are discussed, taking into account recent advances and challenges to hint at potential applications of these approaches in transgenic plant improvement programs. This review also highlights challenges in implementing the nanoparticle-based approaches used in plant genetic engineering. A new technology that improves gene transformation efficiency and overcomes difficulties in plant regeneration has been established and will be used for the de novo production of transgenic plants, and CRISPR/Cas9 genome editing has accelerated crop improvement. Therefore, we outline future perspectives based on combinations of genome editing, nanoparticle-mediated gene transformation and de novo regeneration technologies to accelerate crop improvement. The information provided here will assist an effective exploration of the technological advances in plant genetic engineering to support plant breeding and important crop improvement programs.

Keywords: CRISPR/Cas9; de novo regeneration; nanoparticle; plant genetic engineering; transgenic.

PubMed Disclaimer

References

REFERENCES

    1. Ahmed, M. (2017) Peptides, polypeptides and peptide-polymer hybrids as nucleic acid carriers. Biomater. Sci. 5, 2188-2211.
    1. Balážová, M., Baláž, M. and Babula, P. (2020) Zinc oxide nanoparticles damage tobacco BY-2 cells by oxidative stress followed by processes of autophagy and programmed cell death. Nanomaterials (Basel), 10, 1066.
    1. Banerjee, K., Pramanik, P., Maity, A., Joshi, D.C., Wani, S.H. and Krishnan, P. (2019) Methods of using nanomaterials to plant systems and their delivery to plants (Mode of entry, uptake, translocation, accumulation, biotransformation and barriers), pp. 123-152.
    1. Betekhtin, A., Hus, K., Rojek-Jelonek, M., Kurczynska, E., Nibau, C., Doonan, J.H. and Hasterok, R. (2020) In vitro tissue culture in brachypodium: Applications and challenges. Int. J. Mol. Sci. 21, 1037.
    1. Billingsley, M., Singh, N., Ravikumar, P., Zhang, R., June, C.H. and Mitchell, M.J. (2020) Ionizable lipid nanoparticle mediated mRNA delivery for human CAR t cell engineering. Nano Lett. 20, 1578-1589.

Publication types

LinkOut - more resources