Low Utility of Repeat Real-Time PCR Testing for SARS-CoV-2 in Clinical Specimens

doi:10.1016/j.mayocp.2020.06.020

. 2020 Sep;95(9):1942-1945.

doi: 10.1016/j.mayocp.2020.06.020. Epub 2020 Jul 4.

Low Utility of Repeat Real-Time PCR Testing for SARS-CoV-2 in Clinical Specimens

Douglas W Challener¹, Aditya Shah², John C O'Horo³, Elie Berbari², Matthew J Binnicker⁴, Aaron J Tande²

Affiliations

¹ Division of Infectious Disease, Mayo Clinic, Rochester, MN. Electronic address: challener.douglas@mayo.edu.
² Division of Infectious Disease, Mayo Clinic, Rochester, MN.
³ Division of Infectious Disease, Mayo Clinic, Rochester, MN; Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Rochester, MN.
⁴ Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.

PMID: 32861337
PMCID: PMC7334934
DOI: 10.1016/j.mayocp.2020.06.020

Low Utility of Repeat Real-Time PCR Testing for SARS-CoV-2 in Clinical Specimens

Douglas W Challener et al. Mayo Clin Proc. 2020 Sep.

. 2020 Sep;95(9):1942-1945.

doi: 10.1016/j.mayocp.2020.06.020. Epub 2020 Jul 4.

Authors

Douglas W Challener¹, Aditya Shah², John C O'Horo³, Elie Berbari², Matthew J Binnicker⁴, Aaron J Tande²

Affiliations

¹ Division of Infectious Disease, Mayo Clinic, Rochester, MN. Electronic address: challener.douglas@mayo.edu.
² Division of Infectious Disease, Mayo Clinic, Rochester, MN.
³ Division of Infectious Disease, Mayo Clinic, Rochester, MN; Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Rochester, MN.
⁴ Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN.

PMID: 32861337
PMCID: PMC7334934
DOI: 10.1016/j.mayocp.2020.06.020

Abstract

In a multicenter cohort of 22,315 patients tested for COVID-19, 1676 (7.5%) had repeat testing via real-time polymerase chain reaction following an initial negative test. Of those retested within 7 days of their first negative test, only 2.0% had a positive result. This suggests that repeat testing from the same source is unlikely to provide additional information.

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Comment in

Repeated Testing in SARS-CoV-2 Infection.
Lippi G, Sanchis-Gomar F, Henry BM. Lippi G, et al. Mayo Clin Proc. 2020 Oct;95(10):2283-2284. doi: 10.1016/j.mayocp.2020.08.007. Epub 2020 Aug 10. Mayo Clin Proc. 2020. PMID: 33012357 Free PMC article. No abstract available.
In Reply-Repeated Testing in SARS-CoV-2 Infection.
Shah A, O'Horo JC, Berbari EF, Tande A, Challener D, Binnicker MJ. Shah A, et al. Mayo Clin Proc. 2020 Oct;95(10):2284-2285. doi: 10.1016/j.mayocp.2020.08.006. Epub 2020 Aug 10. Mayo Clin Proc. 2020. PMID: 33012358 Free PMC article. No abstract available.

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Clinical characteristics of hospitalized patients with false-negative severe acute respiratory coronavirus virus 2 (SARS-CoV-2) test results.
MacKenzie EL, Hareza DA, Collison MW, Czapar AE, Kraft AK, Waxse BJ, Friedman EE, Ridgway JP. MacKenzie EL, et al. Infect Control Hosp Epidemiol. 2022 Apr;43(4):467-473. doi: 10.1017/ice.2021.146. Epub 2021 Apr 19. Infect Control Hosp Epidemiol. 2022. PMID: 33867000 Free PMC article.
Repeated Testing in SARS-CoV-2 Infection.
Lippi G, Sanchis-Gomar F, Henry BM. Lippi G, et al. Mayo Clin Proc. 2020 Oct;95(10):2283-2284. doi: 10.1016/j.mayocp.2020.08.007. Epub 2020 Aug 10. Mayo Clin Proc. 2020. PMID: 33012357 Free PMC article. No abstract available.
One-step and sequential SARSCOV-2 polymerase chain reaction tests would not work every time.
Hatami F, Rabiei MM, Javandoust Gharehbagh F, Pourhoseingholi MA, Sabeti S, Kheyrian M, Alavi Darazam I. Hatami F, et al. J Clin Lab Anal. 2022 Feb;36(2):e24226. doi: 10.1002/jcla.24226. Epub 2022 Jan 8. J Clin Lab Anal. 2022. PMID: 34997789 Free PMC article.
Digital Health Surveillance Strategies for Management of Coronavirus Disease 2019.
Jose T, Warner DO, O'Horo JC, Peters SG, Chaudhry R, Binnicker MJ, Burger CD. Jose T, et al. Mayo Clin Proc Innov Qual Outcomes. 2021 Feb;5(1):109-117. doi: 10.1016/j.mayocpiqo.2020.12.004. Epub 2020 Dec 14. Mayo Clin Proc Innov Qual Outcomes. 2021. PMID: 33521582 Free PMC article.
Impact of the Coronavirus Disease 2019 (COVID-19) Vaccine on Asymptomatic Infection Among Patients Undergoing Preprocedural COVID-19 Molecular Screening.
Tande AJ, Pollock BD, Shah ND, Farrugia G, Virk A, Swift M, Breeher L, Binnicker M, Berbari EF. Tande AJ, et al. Clin Infect Dis. 2022 Jan 7;74(1):59-65. doi: 10.1093/cid/ciab229. Clin Infect Dis. 2022. PMID: 33704435 Free PMC article.

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References

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1. Winichakoon P., Chaiwarith R., Liwsrisakun C. Negative nasopharyngeal and oropharyngeal swab does not rule out COVID-19. J Clin Microbiol. 2020;57(5):e00297–e00320. - PMC - PubMed
1. West C.P., Montori V.M., Sampathkumar P. COVID-19 testing: the threat of false-negative results. Mayo Clin Proc. 2020;95(6):1127–1129. - PMC - PubMed
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[1] Fang Y., Zhang H., Xie J. Sensitivity of chest CT for COVID-19: comparison to RT-PCR. Radiology. 2020. Feb 19; 200432. https://doi.org/10.1148/radiol.2020200432 [Epub ahead of print] - DOI - PMC - PubMed

[2] Fang Y., Zhang H., Xie J. Sensitivity of chest CT for COVID-19: comparison to RT-PCR. Radiology. 2020. Feb 19; 200432. https://doi.org/10.1148/radiol.2020200432 [Epub ahead of print] - DOI - PMC - PubMed

[3] Winichakoon P., Chaiwarith R., Liwsrisakun C. Negative nasopharyngeal and oropharyngeal swab does not rule out COVID-19. J Clin Microbiol. 2020;57(5):e00297–e00320. - PMC - PubMed

[4] Winichakoon P., Chaiwarith R., Liwsrisakun C. Negative nasopharyngeal and oropharyngeal swab does not rule out COVID-19. J Clin Microbiol. 2020;57(5):e00297–e00320. - PMC - PubMed

[5] West C.P., Montori V.M., Sampathkumar P. COVID-19 testing: the threat of false-negative results. Mayo Clin Proc. 2020;95(6):1127–1129. - PMC - PubMed

[6] West C.P., Montori V.M., Sampathkumar P. COVID-19 testing: the threat of false-negative results. Mayo Clin Proc. 2020;95(6):1127–1129. - PMC - PubMed

[7] He X., Lau E.H.Y., Wu P. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med. 2020: [Epub ahead of print] Available at: - DOI - PubMed

[8] He X., Lau E.H.Y., Wu P. Temporal dynamics in viral shedding and transmissibility of COVID-19. Nat Med. 2020: [Epub ahead of print] Available at: - DOI - PubMed

[9] Zou L., Ruan F., Huang M. SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med. 2020;382:1177–1179. - PMC - PubMed

[10] Zou L., Ruan F., Huang M. SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med. 2020;382:1177–1179. - PMC - PubMed

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Low Utility of Repeat Real-Time PCR Testing for SARS-CoV-2 in Clinical Specimens

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Low Utility of Repeat Real-Time PCR Testing for SARS-CoV-2 in Clinical Specimens

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