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. 2020 Aug 27;9(9):1102.
doi: 10.3390/plants9091102.

Effects of Green Brazilian Propolis Alcohol Extract on Nociceptive Pain Models in Rats

Mohammed T Al-Hariri  1 , Turki S Abualait  2
Affiliations

Effects of Green Brazilian Propolis Alcohol Extract on Nociceptive Pain Models in Rats

Mohammed T Al-Hariri et al. Plants (Basel). .

Abstract

Pain is one of the most common symptoms encountered in the medical practice. None of the management procedures used currently offer a complete relief for patients suffering from nociceptive pain. New treatment strategies for pain management are needed. Propolis has been used in traditional medicine to relieve various types of pain. The aim of the current study was to investigate the potential effects of the green Brazilian propolis alcohol extract in vivo on the nociceptive and inflammatory pain models in rats. Rats were distributed into three random groups (n = 6); Group I: control group received normal saline intraperitoneally (i.p.); Group II: treated with green Brazilian propolis alcohol extract (P50 mg/kg i.p.); Group III: treated with P100 mg/kg i.p. After sixty minutes, 50 μL of 5% formalin was injected subcutaneously into the dorsal surface of the right hind paw. The nociceptive response was identified by counting the number of flinches of the injected paw. The number of flinches was counted for the period of 0-5 min (early phase; neurogenic) and 10-60 min (late phase; inflammatory). Thermal hyperalgesia was assessed using three-paw withdrawal latency measurement with ten minutes intervals using a planter analgesic meter. Abdominal writhe (contraction) was induced by i.p. injection of acetic acid (1 mL of 2%). The results showed that green Brazilian propolis alcohol extract caused a significant inhibition of acetic acid-induced pain and significantly increased the pain threshold against infrared and formalin tests. The promising antinociceptive and anti-inflammatory properties of propolis and/or its active constituents as natural compounds in the present study indicates that it merits further studies in pain.

Keywords: acetic acid; antinociceptive pain; formalin test; propolis; rats.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Antinociceptive effect of green Brazilian propolis alcohol extract (P50 and P100) in the infrared test in the rats. The values expressed are mean ± SEM, (n = 6).* p < 0.05: significantly different for treated (P50 and P100) compared to the control groups as determined by ANOVA analysis followed by multiple comparison post hoc test.
Figure 2
Figure 2
Effect of green Brazilian propolis alcohol extract (P50 and P100) in the formalin-induced nociception model: neurogenic (Phase I) and inflammatory (Phase II). The values expressed are mean ± SEM, (n = 6). * p < 0.05: significantly different for treated (P50 and P100) compared to the control groups as determined by ANOVA analysis followed by multiple comparison post hoc test.
Figure 3
Figure 3
Effect of green Brazilian propolis alcohol extract (P50 and P100) against acetic acid-induced abdominal contraction in rats. The values expressed are mean ± SEM (n = 6).* p < 0.05: significantly different for treated (P50 and P100) compared to the control groups as determined by ANOVA analysis followed by multiple comparison post hoc test.
See this image and copyright information in PMC

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