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Review
. 2020 Aug 27;10(9):348.
doi: 10.3390/metabo10090348.

Bridging Targeted and Untargeted Mass Spectrometry-Based Metabolomics via Hybrid Approaches

Li Chen  1   2 , Fanyi Zhong  3 , Jiangjiang Zhu  1   2
Affiliations
Review

Bridging Targeted and Untargeted Mass Spectrometry-Based Metabolomics via Hybrid Approaches

Li Chen et al. Metabolites. .

Abstract

This mini-review aims to discuss the development and applications of mass spectrometry (MS)-based hybrid approaches in metabolomics. Several recently developed hybrid approaches are introduced. Then, the overall workflow, frequently used instruments, data handling strategies, and applications are compared and their pros and cons are summarized. Overall, the improved repeatability and quantitative capability in large-scale MS-based metabolomics studies are demonstrated, in comparison to either targeted or untargeted metabolomics approaches alone. In summary, we expect this review to serve as a first attempt to highlight the development and applications of emerging hybrid approaches in metabolomics, and we believe that hybrid metabolomics approaches could have great potential in many future studies.

Keywords: broad metabolite coverage; dynamic range; hybrid approaches; identification; metabolomics; quantitative analysis; repeatability.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The workflow demonstrates the development process from targeted metabolomics and untargeted metabolomics to novel metabolomics analysis. Note: HRMS is commonly used in untargeted metabolomics; however, it could also be used in targeted metabolomics.
Figure 2
Figure 2
The workflow of novel metabolomics analysis with hybrid approaches [26,27,28,29].
Figure 3
Figure 3
Considerations for novel metabolomics analysis with hybrid approaches with advantages from both untargeted and targeted analysis.
See this image and copyright information in PMC

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