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. 2020 Sep-Oct;34(5):2475-2484.
doi: 10.21873/invivo.12063.

The Effects of Ascorbic Acid and U-74389G on Renal Ischemia-Reperfusion Injury in a Rat Model

Constantinos G Zografos #  1   2 , Dimosthenis Chrysikos #  1   2 , Theodoros Pittaras  1   3 , Vasileios Karampelias  4 , Aikaterini Chairakakis  1 , Antonis Galanos  1 , Ioannis Sfiniadakis  5 , Evangelos Felekouras  6 , George C Zografos  2 , Michail Sideris  7 , Konstantina Papadopoulou  1 , Apostolos E Papalois  8   9
Affiliations

The Effects of Ascorbic Acid and U-74389G on Renal Ischemia-Reperfusion Injury in a Rat Model

Constantinos G Zografos et al. In Vivo. 2020 Sep-Oct.

Abstract

Background/aim: U-74389G and ascorbic acid protect the cells from oxidation. This study aimed to depict their role in ischemia-reperfusion injury in a renal rat model.

Materials and methods: Sixty Wistars rats were randomized into six groups of 10 animals each. Group A Ischemia 30 min, reperfusion 60 min; Group B Ischemia 30 min, reperfusion 120 min; Group C Ischemia 30 min, ascorbic acid administration, reperfusion 60 min; Group D Ischemia 30 min, ascorbic acid administration, reperfusion 120 min; Group E Ischemia 30 min, U-74389G administration, reperfusion 60 min; Group F Ischemia 30 min, U-74389G administration, reperfusion 120 min. We then collected tissue and blood samples.

Results: Histology and the significantly decreased malondialdehyde and tumor necrosis factor-α levels indicated that ascorbic acid was superior to U-74389G, at pre-defined time intervals.

Conclusion: Ascorbic acid and U-74389G ameliorated renal damage induced by ischemia-reperfusion injury, suggesting a therapeutic effect.

Keywords: Antioxidants; ascorbic acid; kidney; reperfusion injury.

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Conflict of interest statement

The Authors declare no conflicts of interest regarding this study.

Figures

Figure 1
Figure 1. Main variables among the groups expressed as MEAN+SD after 60 min and 120 min of reperfusion.
Figure 2
Figure 2. Main variables among the groups expressed as MEAN+SD after 60 min and 120 min of reperfusion.
Figure 3
Figure 3. No statistically significant difference in TNF-α between the compared groups at 60 min reperfusion. U-74389G group presents statistically significantly higher values of TNF-α compared to the ascorbic acid group (p=0.049) at 120 min reperfusion.
Figure 4
Figure 4. Vascular dilatation and congestion with areas of necrosis (250×). Group C (kindly ischemia (30 min), ascorbic acid administration, followed by reperfusion for 60 min).
Figure 5
Figure 5. Vascular dilatation and congestion with areas of necrosis (400×). Group C [kidney ischemia (30 min), ascorbic acid administration, followed by reperfusion for 60 min].
Figure 6
Figure 6. Normal tubular architecture of the kidney (400×). Group E (kidney ischemia (30 min), U-74389G intravenous injection, and reperfusion for 60 min).
Figure 7
Figure 7. Isolated degenerated renal tubules mainly peripherally. Group E [kidney ischemia (30 min), U-74389G intravenous injection, and reperfusion for 60 min].
Figure 8
Figure 8. Few degenerated renal tubules with central distribution (250×). Group F [kidney ischemia (30 min), U-74389G intravenous injection, and reperfusion for 120 min].
See this image and copyright information in PMC

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