Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Sep-Oct;34(5):2507-2516.
doi: 10.21873/invivo.12067.

Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart

Savas Ustunova  1 , Selcuk Takir  2 , Nadim Yilmazer  3 , Huri Bulut  4 , Didem Altindirek  5 , Ozden Hatirnaz Ng  6 , Cihan Demirci Tansel  7 , B Sonmez Uydes Dogan  8 , Ugur Ozbek  9 , Elif Ilkay Armutak  10 , Ebru Gurel Gurevin  7
Affiliations

Hydrogen Sulphide and Nitric Oxide Cooperate in Cardioprotection Against Ischemia/Reperfusion Injury in Isolated Rat Heart

Savas Ustunova et al. In Vivo. 2020 Sep-Oct.

Abstract

Background/aim: This study was designed to provide further evidence for the interactions between hydrogen sulfide (H2S) and nitric oxide (NO) in ischemia/reperfusion (I/R) injury.

Materials and methods: Rat hearts were studied with the Langendorff technique using the H2S donor sodium hydrosulfide (NaHS, 40 μM) and the cystathionine gamma-lyase (CTH or CSE) inhibitor DL-propargylglycine (PAG, 1 mM). NO synthase inhibitor L-NG-nitroarginine methyl ester (L-NAME, 30 mg/kg, 7 days) was administered before the isolation. The hearts were homogenized for biochemical and molecular analysis.

Results: NaHS reversed I/R-induced cardiac performance impairment, increased tissue nitric oxide production and decreased tissue markers for cardiac injury, while L-NAME inhibited these effects. The expression of CTH was increased with PAG, which was suppressed by L-NAME.

Conclusion: H2S and NO increase each other's production suggesting their interaction and cooperation in cardioprotection against I/R injury.

Keywords: Hydrogen sulfide; ischemia/reperfusion injury; isolated heart; nitric oxide; oxidative damage.

PubMed Disclaimer

Conflict of interest statement

The Authors declare that there are no conflicts of interest associated with this work.

Figures

Figure 1
Figure 1. Experimental design (↓: time points for cardiodynamic analyses).
Figure 2
Figure 2. Cardiodynamic analysis. (A) The end diastolic pressure (EDP), B) Left ventricular developed pressure (LVDP) values of all groups. *p<0.05, **p<0.01, ***p<0.001, statistical significance compared to the IR group; +p<0.05, ++p<0.01, +++p<0.001, statistical significance compared to the L-NAME group.
Figure 3
Figure 3. Cardiodynamic analysis. A) The Max dP/dt, B) Rate pressure product (RPP) values of all groups. *p<0.05, **p<0.01, ***p<0.001, statistical significance compared to the IR group; +p<0.05, ++p<0.01, +++p<0.001, statistical significance compared to the L-NAME group.
Figure 4
Figure 4. Biochemical analysis in heart tissue samples. A) CK-MB, B) LDH, C) GPx, D) H2S and E) Nitrate/nitrite levels of all groups. **p<0.01, ***p<0.001, statistical significance compared to the IR group; +p<0.05, ++p<0.01, +++p<0.001, statistical significance compared to the L-NAME group.
Figure 5
Figure 5. RT-PCR analysis in heart tissue samples. A) eNOS B) iNOS and C) Cth mRNA expressions. All expression levels were measured relative to β-actin by RT-PCR. **p<0.01, statistical significance compared to the PAG group.
See this image and copyright information in PMC

References

    1. Wang R. Two’s company, three’sa crowd: Can H2S be the third endogenous gaseous transmitter. FASEB J. 2002;16(13):1792–1798. doi: 10.1096/fj.02-0211hyp. - DOI - PubMed
    1. Bełtowski J. Hydrogen sulfide in pharmacology and medicine–an update. Pharmacol Rep. 2015;67(3):647–658. doi: 10.1016/j.pharep.2015.01.005. - DOI - PubMed
    1. Estienne A, Portela VM, Choi Y, Zamberlam G, Boerboom D, Roussel V, Meinsohn M-C, Brännström M, Curry TE Jr., Jo M. The endogenous hydrogen sulfide generating system regulates ovulation. Free Radic Biol Med. 2019;138:43–52. doi: 10.1016/j.freeradbiomed.2019.03.028. - DOI - PubMed
    1. Nicholson CK, Calvert JW. Hydrogen sulfide and ischemia–reperfusion injury. Pharmacol Res. 2010;62(4):289–297. doi: 10.1016/j.phrs.2010.06.002. - DOI - PMC - PubMed
    1. Shefa U, Yeo SG, Kim M-S, Song IO, Jung J, Jeong NY, Huh Y. Role of gasotransmitters in oxidative stresses, neuroinflammation, and neuronal repair. Biomed Res Int. 2017;2017 doi: 10.1155/2017/1689341. - DOI - PMC - PubMed