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. 2020 Aug 25;10(27):200906.
doi: 10.5696/2156-9614-10.27.200906. eCollection 2020 Sep.

Effect of Air Pollution on Glutathione S-Transferase Activity and Total Antioxidant Capacity: Cross Sectional Study in Kuwait

Abeer M Almutairi  1 , Yazan Akkam  2 , Mohammad F Alajmi  3 , Nosaibah Akkam  2
Affiliations

Effect of Air Pollution on Glutathione S-Transferase Activity and Total Antioxidant Capacity: Cross Sectional Study in Kuwait

Abeer M Almutairi et al. J Health Pollut. .

Abstract

Background: Air pollution poses a significant threat to human health worldwide. Investigating potential health impacts is essential to the development of regulations and legislation to minimize health risks.

Objectives: The aim of the present study was to investigate the potentially hazardous effect of air pollution on the Ali Sabah Al Salem residential area in Kuwait by comparing the pollution level to a control area (Al-Qirawan) by assessing two biomarkers: erythrocyte glutathione S-transferases (e-GST) and total blood antioxidant, and then correlating the activity to pollution-related oxidative stress.

Methods: The average concentrations of several airborne gases were measured at Ali Sabah Al Salem and Al-Qirawan, including ozone, carbon monoxide, nitrogen dioxide, nitrogen oxides, particulate matter less than 10 μm (PM10), sulfur dioxide, ammonia, carbon dioxide, hydrogen sulfide, methane, and non-methane hydrocarbon. A total of fifty-eight participants were sampled from two different areas and divided into two groups. The study group was composed of 40 residents exposed to polluted ambient air in the Ali Sabah Al Salem residential area. A reference group composed of 18 residents in the Al-Qairawan area living far from major pollution sources was also tested.

Results: All measured gases were higher in concentration at Ali Sabah Al Salem compared to the Al-Qirawan area. Furthermore, PM10 and sulfur dioxide were higher than World Health Organization (WHO) guidelines. The e-GST activity was lower among participants of the Ali Sabah Al Salem residential area compared to participants living in the Al-Qairawan area. The total antioxidant capacity in whole blood of Ali Sabah Al Salem residents was significantly (p<0.0001) higher than in control subjects.

Conclusions: Residents in Ali Sabah Al Salem are exposed to a high level of air pollution that has a serious impact on glutathione S-transferases levels. Subsequently, regulations on pollution sources are needed to lower current health risks. Furthermore, the present study provides evidence that finger-prick blood sampling is a quick, non-invasive method suitable for screening e-GST activity and total antioxidants which may be applied for surveillance purposes.

Participant consent: Obtained.

Ethics approval: The study was approved by the Scientific Research Committee of the Public Authority for Applied Education and Training, Kuwait.

Competing interests: The authors declare no competing financial interests.

Keywords: 1-chloro-2; 4-dinitrobenzene; air-pollution; glutathione S-transferase; oxidative stress; total antioxidant.

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Figures

Figure 1
Figure 1
Geographic location of the study area. A) Map of Kuwait showing Al-Qairawan area (yellow shade), and Ali Sabah Al Salem residential area (red shade). B) Detailed map showing Al-Qairawan area (yellow shade), and Ali Sabah Al Salem residential area (red shade) along with possible sources of pollution.
Figure 2
Figure 2
The increase of air pollution in Ali Sabah Al Salem over Al-Qairawan
Figure 3
Figure 3
Comparison of e-GST activity between Ali Sabah Al Salem and Al-Qairawan. Error bars represent the standard deviation.
See this image and copyright information in PMC

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