Structure of SARS-CoV-2 main protease in the apo state

Xuelan Zhou^#¹, Fanglin Zhong^#^{2

3}, Cheng Lin^{4

5}, Xiaohui Hu¹, Yan Zhang⁶, Bing Xiong⁷, Xiushan Yin^{8

9}, Jinheng Fu¹⁰, Wei He², Jingjing Duan¹¹, Yang Fu¹², Huan Zhou¹³, Peter J McCormick¹⁴, Qisheng Wang¹⁵, Jian Li^{16

17}, Jin Zhang¹⁸

Affiliations

¹ School of Basic Medical Sciences, Nanchang University, Nanchang, 330031, China.
² College of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, 341000, China.
³ Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, 341000, China.
⁴ Shenzhen Crystalo Biopharmaceutical Co., Ltd, Shenzhen, 518118, China.
⁵ Jiangxi Jmerry Biopharmaceutical Co., Ltd, Ganzhou, 341000, China.
⁶ The Second Affiliated Hospital of Nanchang University, Nanchang, 330031, China.
⁷ Department of Medicinal Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
⁸ Applied Biology Laboratory, Shenyang University of Chemical Technology, Shenyang, 110142, China.
⁹ Biotech & Biomedicine Science (Jiangxi) Co. Ltd, Ganzhou, 341000, China.
¹⁰ Jiangxi-OAI Joint Research Institution, Nanchang University, Nanchang, 330047, China.
¹¹ Human Aging Research Institute (HARI), School of Life Sciences, Nanchang University, Nanchang, 330031, China.
¹² School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China.
¹³ Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201204, China.
¹⁴ Centre for Endocrinology, William Harvey Research Institute, Bart's and the London School of Medicine and Dentistry, Queen Mary, University of London, London, EC1M 6BQ, UK.
¹⁵ Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201204, China. wangqisheng@zjlab.org.cn.
¹⁶ College of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, 341000, China. rmsl_2040@163.com.
¹⁷ Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, 341000, China. rmsl_2040@163.com.
¹⁸ School of Basic Medical Sciences, Nanchang University, Nanchang, 330031, China. zhangxiaokong@hotmail.com.

^# Contributed equally.

PMID: 32880863
PMCID: PMC7471536
DOI: 10.1007/s11427-020-1791-3

Comment

Structure of SARS-CoV-2 main protease in the apo state

Xuelan Zhou et al. Sci China Life Sci. 2021 Apr.

. 2021 Apr;64(4):656-659.

doi: 10.1007/s11427-020-1791-3. Epub 2020 Sep 1.

Authors

Affiliations

¹ School of Basic Medical Sciences, Nanchang University, Nanchang, 330031, China.
² College of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, 341000, China.
³ Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, 341000, China.
⁴ Shenzhen Crystalo Biopharmaceutical Co., Ltd, Shenzhen, 518118, China.
⁵ Jiangxi Jmerry Biopharmaceutical Co., Ltd, Ganzhou, 341000, China.
⁶ The Second Affiliated Hospital of Nanchang University, Nanchang, 330031, China.
⁷ Department of Medicinal Chemistry, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.
⁸ Applied Biology Laboratory, Shenyang University of Chemical Technology, Shenyang, 110142, China.
⁹ Biotech & Biomedicine Science (Jiangxi) Co. Ltd, Ganzhou, 341000, China.
¹⁰ Jiangxi-OAI Joint Research Institution, Nanchang University, Nanchang, 330047, China.
¹¹ Human Aging Research Institute (HARI), School of Life Sciences, Nanchang University, Nanchang, 330031, China.
¹² School of Medicine, Southern University of Science and Technology, Shenzhen, 518055, China.
¹³ Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201204, China.
¹⁴ Centre for Endocrinology, William Harvey Research Institute, Bart's and the London School of Medicine and Dentistry, Queen Mary, University of London, London, EC1M 6BQ, UK.
¹⁵ Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201204, China. wangqisheng@zjlab.org.cn.
¹⁶ College of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, 341000, China. rmsl_2040@163.com.
¹⁷ Laboratory of Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Ministry of Education, Gannan Medical University, Ganzhou, 341000, China. rmsl_2040@163.com.
¹⁸ School of Basic Medical Sciences, Nanchang University, Nanchang, 330031, China. zhangxiaokong@hotmail.com.

^# Contributed equally.

PMID: 32880863
PMCID: PMC7471536
DOI: 10.1007/s11427-020-1791-3

No abstract available

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Comment on

Structure-based design of antiviral drug candidates targeting the SARS-CoV-2 main protease.
Dai W, Zhang B, Jiang XM, Su H, Li J, Zhao Y, Xie X, Jin Z, Peng J, Liu F, Li C, Li Y, Bai F, Wang H, Cheng X, Cen X, Hu S, Yang X, Wang J, Liu X, Xiao G, Jiang H, Rao Z, Zhang LK, Xu Y, Yang H, Liu H. Dai W, et al. Science. 2020 Jun 19;368(6497):1331-1335. doi: 10.1126/science.abb4489. Epub 2020 Apr 22. Science. 2020. PMID: 32321856 Free PMC article.
Structural basis for the inhibition of SARS-CoV-2 main protease by antineoplastic drug carmofur.
Jin Z, Zhao Y, Sun Y, Zhang B, Wang H, Wu Y, Zhu Y, Zhu C, Hu T, Du X, Duan Y, Yu J, Yang X, Yang X, Yang K, Liu X, Guddat LW, Xiao G, Zhang L, Yang H, Rao Z. Jin Z, et al. Nat Struct Mol Biol. 2020 Jun;27(6):529-532. doi: 10.1038/s41594-020-0440-6. Epub 2020 May 7. Nat Struct Mol Biol. 2020. PMID: 32382072

References

1. Dai W, Zhang B, Jiang XM, Su H, Li J, Zhao Y, Xie X, Jin Z, Peng J, Liu F, et al. Structure-based design of antiviral drug candidates targeting the SARS-CoV-2 main protease. Science. 2020;368:1331–1335. doi: 10.1126/science.abb4489. - DOI - PMC - PubMed
1. Hilgenfeld R. From SARS to MERS: crystallographic studies on coronaviral proteases enable antiviral drug design. FEBS J. 2014;281:4085–4096. doi: 10.1111/febs.12936. - DOI - PMC - PubMed
1. Jin Z, Du X, Xu Y, Deng Y, Liu M, Zhao Y, Zhang B, Li X, Zhang L, Peng C, et al. Structure of Mpro from SARS-CoV-2 and discovery of its inhibitors. Nature. 2020;582:289–293. doi: 10.1038/s41586-020-2223-y. - DOI - PubMed
1. Jin Z, Zhao Y, Sun Y, Zhang B, Wang H, Wu Y, Zhu Y, Zhu C, Hu T, Du X, et al. Structural basis for the inhibition of SARS-CoV-2 main protease by antineoplastic drug carmofur. Nat Struct Mol Biol. 2020;27:529–532. doi: 10.1038/s41594-020-0440-6. - DOI - PubMed
1. Lu R, Zhao X, Li J, Niu P, Yang B, Wu H, Wang W, Song H, Huang B, Zhu N, et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020;395:565–574. doi: 10.1016/S0140-6736(20)30251-8. - DOI - PMC - PubMed

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Structure of SARS-CoV-2 main protease in the apo state

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Structure of SARS-CoV-2 main protease in the apo state

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