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. 2020 Sep 3;10(1):14547.
doi: 10.1038/s41598-020-71442-8.

Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment

Faiz Ur Rahman  1   2 , Karl B Andree  3 , Nuria Salas-Massó  3   4 , Margarita Fernandez-Tejedor  3 , Anna Sanjuan  3 , Maria J Figueras  4 , M Dolors Furones  3
Affiliations

Improved culture enrichment broth for isolation of Arcobacter-like species from the marine environment

Faiz Ur Rahman et al. Sci Rep. .

Abstract

Arcobacter-like species are found associated with many matrices, including shellfish in marine environments. The culture media and conditions play a major role in the recovery of new Arcobacter-like species. This study was aimed to develop a culture media for isolation and enhanced growth of Arcobacter-like spp. from marine and shellfish matrices. For this purpose, 14 different Arcobacter-like spp. mostly isolated from shellfish, were grown in 24 different formulations of enrichment broths. The enrichment broths consisted of five main groups based on the organic contents (fresh oyster homogenate, lyophilized oyster either alone or in combination with other standard media), combined with artificial seawater (ASW) or 2.5% NaCl. Optical density (OD420nm) measurements after every 24 h were compared with the growth in control media (Arcobacter broth) in parallel. The mean and standard deviation were calculated for each species in each broth and statistical differences (p < 0.05) among broths were calculated by ANOVA. The results indicated that shellfish-associated Arcobacter-like species growth was significantly higher in Arcobacter broth + 50% ASW and the same media supplemented with lyophilized oysters. This is the first study to have used fresh or lyophilized oyster flesh in the enrichment broth for isolation of shellfish-associated Arcobacter-like spp.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Growth of Arcobacter-like spp. in the conventional broth media. AB = Arcobacter Broth (Control Enrichment Broth); MB = Marine Broth; HI = Heart Infusion; ABNC = Arcobacter Broth + 2.5% NaCl; ABSW = Arcobacter Broth + 50% ASW; ABSW100 = Arcobacter Broth + 100% ASW. (Note: The strains with a T in the last are Type strains).
Figure 2
Figure 2
Growth of Arcobacter-like spp. in the conventional broth media supplemented with oyster homogenate. AB = Arcobacter Broth (Control Enrichment Broth); ABOH = Arcobacter Broth + Oyster Homogenate; ABOHN = Arcobacter Broth + Oyster Homogenate + 2.5% NaCl; ABOHSW = Arcobacter Broth + Oyster Homogenate + 50% ASW; ABSW100 = Arcobacter Broth + Oyster Homogenate + 100% ASW; MBOH = Marine Broth + Oyster Homogenate. (Note: The strains with a “T” suffix are Type strains).
Figure 3
Figure 3
Growth of Arcobacter-like spp. in the enrichment broth media prepared from oyster homogenate. AB = Arcobacter Broth (Control Enrichment Broth); OH = Oyster Homogenate + Distilled H2O; OHNC = Oyster Homogenate + 2.5% NaCl; OHSW = Oyster Homogenate + 50% ASW; OHSW100 = Oyster Homogenate + 100% ASW. (Note: The strains with a “T” suffix are Type strains).
Figure 4
Figure 4
Growth of Arcobacter-like spp. in conventional broth media supplemented with lyophilized oyster. AB = Arcobacter Broth (Control Enrichment Broth); ABL = Arcobacter Broth + Lyophilized Oyster; ABLNC = Arcobacter Broth + Lyophilized Oyster + 2.5% NaCl; ABLSW = Arcobacter Broth + Lyophilized Oyster + 50% ASW; ABLSW100 = Arcobacter Broth + Lyophilized Oyster + 100% ASW; MBL = Marine Broth + Lyophilized Oyster. (Note: The strains with a “T” suffix are Type strains).
Figure 5
Figure 5
Growth of Arcobacter-like spp. in the broth media prepared with lyophilized oyster. AB = Arcobacter Broth (Control Enrichment Broth); LO = Lyophilized Oyster + Distilled H2O; LONC = Lyophilized Oyster + 2.5% NaCl; LOSW = Lyophilized Oyster + 50% ASW; LOSW100 = Lyophilized Oyster + 100% ASW. (Note: The strains with a “T” suffix are Type strains).
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