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. 2020 Sep;27(9):2439-2443.
doi: 10.1016/j.sjbs.2020.06.046. Epub 2020 Jul 2.

Molecular & biochemical analysis of Pro12Ala variant of PPAR-γ2 gene in type 2 diabetes mellitus

Rabbani Syed  1 , Kaiser Jamil  2 , M Asimuddin  2 , Mohammed S Alqahtani  1 , Meshal Alshehri  3 , Ayesha Mateen  3 , Abdul Wahab Ali Aduderman  4 , Mohammad Shamsul Ola  5 , Abdul Malik  1
Affiliations

Molecular & biochemical analysis of Pro12Ala variant of PPAR-γ2 gene in type 2 diabetes mellitus

Rabbani Syed et al. Saudi J Biol Sci. 2020 Sep.

Abstract

Diabetes has emerged as a major threat to human life globally. Genomic studies have found a significant link between the Pro12Ala polymorphism of the PPAR-γ2 gene with incidence as well as occurrence of the risk of metabolic syndrome. The present study was aimed at assessing the PPAR-γ2 variant in an Asian Indian cohort of type 2 diabetes patients and its correlation with metabolic parameters. The present case-control study involved 100 type 2 diabetic patients and 100 asymptomatic healthy volunteers enrolled in random. Assessment of demographic factors and biochemical parameters were done for all enrolled. In addition, genotyping for the Pro12Ala (CCA to GCA) polymorphism was done by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) technology. The genotyping study detected the frequency of the CC genotype (Pro12Pro) to be higher in frequency in comparison to the heterozygous CG genotype in both, cases and controls. The homozygous GG genotype (Ala12Ala) was not detected in any of the cases or controls assessed. Biochemical analysis of the levels of malondialdehyde (MDA) detected a significant increase (p < 0.0001). Additionally, increase in levels of fasting and postprandial glucose, total cholesterol, triglycerides, and parameters of the liver and renal function tests were detected. This study detected the PPAR-γ2 to be a significant biomarker for type 2 diabetes mellitus.

Keywords: Asian Indians; PPARG; Polymerase chain reaction; Pro12Ala; T2DM.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
PCR-RFLP results for the Pro12Ala polymorphism. Lane 1 for uncut DNA; lanes 2 and 4 for Pro12Ala heterozygotes; lane 3 for Pro12Pro homozygote; lane 5 for 100 bp marker.
See this image and copyright information in PMC

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