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. 1988 Jun;74(3):366-9.

Field inversion gel electrophoretic separation of Cryptosporidium spp. chromosome-sized DNA

J R Mead  1 , M J Arrowood, W L Current, C R Sterling
Affiliations
  • PMID: 3288738

Field inversion gel electrophoretic separation of Cryptosporidium spp. chromosome-sized DNA

J R Mead et al. J Parasitol. 1988 Jun.

Abstract

Chromosomal DNA from 5 isolates of Cryptosporidium parvum and 1 of C. baileyi were compared by field-inversion gel electrophoresis (FIGE). FIGE analyses of parasite DNA prepared from purified sporozoites versus intact oocysts showed no observable differences. Chromosomal DNA migration patterns of the 5 C. parvum isolates were indistinguishable, whereas similar but distinct differences were evident between C. baileyi and the isolates of C. parvum. Oocyst-reactive monoclonal antibodies differentiated oocysts of C. parvum from those of C. baileyi but were unable to distinguish oocysts of 1 isolate of C. parvum from another.

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