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Comment
. 2020 Dec;52(7):814-816.
doi: 10.1016/j.pathol.2020.08.002. Epub 2020 Aug 19.

Contamination of SARS-CoV-2 RT-PCR probes at the oligonucleotide manufacturer

Claire Y T Wang  1 , Cameron Buckley  2 , Cheryl Bletchly  3 , Patrick Harris  4 , David Whiley  4
Affiliations
Comment

Contamination of SARS-CoV-2 RT-PCR probes at the oligonucleotide manufacturer

Claire Y T Wang et al. Pathology. 2020 Dec.
No abstract available

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Figures

Figure 1
Fig. 1
Illustration of flanking primer designs for detection of contaminated probe. Note that six additional primers were designed for the E-gene but only four for the RdRP assay. FOR, forward primer; REV, reverse primer.
See this image and copyright information in PMC

Comment on

  • Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
    Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DK, Bleicker T, Brünink S, Schneider J, Schmidt ML, Mulders DG, Haagmans BL, van der Veer B, van den Brink S, Wijsman L, Goderski G, Romette JL, Ellis J, Zambon M, Peiris M, Goossens H, Reusken C, Koopmans MP, Drosten C. Corman VM, et al. Euro Surveill. 2020 Jan;25(3):2000045. doi: 10.2807/1560-7917.ES.2020.25.3.2000045. Euro Surveill. 2020. PMID: 31992387 Free PMC article.

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