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. 2020 Oct:177:106043.
doi: 10.1016/j.mimet.2020.106043. Epub 2020 Sep 5.

A quick flow cytometry protocol to assess Helicobacter pylori viability

Claudio Alba  1 , Alicia C Marin  2 , Adrian G McNicholl  3 , Ana Montalban-Arques  3 , Irene Mora-Gutierrez  4 , Antonio José Sánchez-Arroyo  5 , Tamara Soler  1 , David García-Fresnadillo  5 , Javier P Gisbert  3 , Teresa Alarcón  1 , David Bernardo  6
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A quick flow cytometry protocol to assess Helicobacter pylori viability

Claudio Alba et al. J Microbiol Methods. 2020 Oct.

Abstract

Here we present an easy flow cytometry protocol to study the viability of Helicobacter pylori which also enables the detection of even low live bacteria densities. This protocol has potential utility for a fast and accurate assessment of experimental eradication methods against H. pylori.

Keywords: Flow cytometry; Helicobacter pylori; Propidium iodide; Syto 9; Viability.

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Declaration of Competing Interest None.

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