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. 2020 Sep 6;25(18):4069.
doi: 10.3390/molecules25184069.

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

Georgiana-Mădălina Lemnaru Popa  1 , Roxana Doina Truşcă  1 , Cornelia-Ioana Ilie  1 , Roxana Elena Țiplea  1 , Denisa Ficai  1 , Ovidiu Oprea  1 , Anicuța Stoica-Guzun  1 , Anton Ficai  1   2 , Lia-Mara Dițu  3
Affiliations

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

Georgiana-Mădălina Lemnaru Popa et al. Molecules. .

Abstract

The use of bacterial cellulose (BC) in skin wound treatment is very attractive due to its unique characteristics. These dressings' wet environment is an important feature that ensures efficient healing. In order to enhance the antimicrobial performances, bacterial-cellulose dressings were loaded with amoxicillin and bacitracin as antibacterial agents. Infrared characterization and thermal analysis confirmed bacterial-cellulose binding to the drug. Hydration capacity showed good hydrophilicity, an efficient dressing's property. The results confirmed the drugs' presence in the bacterial-cellulose dressing's structure as well as the antimicrobial efficiency against Staphylococcus aureus and Escherichia coli. The antimicrobial assessments were evaluated by contacting these dressings with the above-mentioned bacterial strains and evaluating the growth inhibition of these microorganisms.

Keywords: antimicrobial effect; bacterial cellulose; dressings; drug delivery; skin wounds.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
BC control SEM images, showing the fiber morphology on the surface (A 50,000×; B 10,000×) and in cross-section (C 20,000×; D 10,000×).
Figure 2
Figure 2
FTIR spectra of bacitracin control, BC-bacitracin 1%, BC-bacitracin 3%, and BC control.
Figure 3
Figure 3
FTIR spectra for BC control, BC-amoxicillin 1%, BC-amoxicillin 3%, and amoxicillin control.
Figure 4
Figure 4
Thermal analysis of BC-bacitracin 1%.
Figure 5
Figure 5
Thermal analysis of BC-bacitracin 3%.
Figure 6
Figure 6
Thermal analysis of BC-amoxicillin 1% and 3%.
Figure 7
Figure 7
Absorption kinetics of BC samples loaded with bacitracin 1% and 3%.
Figure 8
Figure 8
Absorption kinetics of BC samples loaded with amoxicillin 1% and 3%.
Figure 9
Figure 9
Graphical representation of CFU/mL values of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, in order to evaluate the capability of the bacterial cells to adhere on the surface of BC-bacitracin samples.
Figure 10
Figure 10
Graphical representation of CFU/mL values of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922, in order to evaluate the bacitracin release from the BC-bacitracin samples into the broth media.
Figure 11
Figure 11
Graphical representation of CFU/mL values of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 in order to evaluate the capability of the bacterial cells to adhere on the surface of BC-amoxicillin samples.
Figure 12
Figure 12
Graphical representation of CFU/mL values of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 in order to evaluate the amoxicillin release from the BC-Amoxicillin samples into the broth media.
Figure 13
Figure 13
The technological process flowchart for manufacturing bacterial cellulose membranes loaded with bacitracin and amoxicillin.
See this image and copyright information in PMC

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