The endocrine cells in the gut of Mugil saliens Risso, 1810 (Teleostei): an immunocytochemical and ultrastructural study

Abstract

The endocrine cells in the gut of Mugil saliens Risso, 1810 (leaping grey mullet) were investigated by immunocytochemical and electron microscopic techniques. Gastrin-, glucagon-, and somatostatin-immunoreactive cells were identified in the cardiac and cecal stomach regions, located mainly in the lower part of the gastric folds and in the upper part of the glands. Substance P-, somatostatin-, and pancreatic polypeptide (PP)-immunoreactive cells were found between epithelial cells in the pyloric stomach region. Gastrin-, cholecystokinin (CCK)-, gastric inhibitory polypeptide (GIP)-, substance P-, Met-enkephalin- and PP-immunoreactive cells were observed throughout the intestine while only the last three of these appeared in the posterior intestine. Nine types of gastroenteroendocrine cells were ultrastructurally characterized; some of them were related to the cell types immunocytochemically identified.