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. 2020 Dec;103(6):2239-2243.
doi: 10.4269/ajtmh.20-0124. Epub 2020 Sep 3.

Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis

Marcelo Rudzinski  1   2   3 , Lais Pardini  3   4 , Mariana Bernstein  3   4 , Gastón Moré  3   4 , Marina Khoury  5 , Silvana Carolina Duarte  2 , Carina Argüelles  6 , Silvia Reina  2   3 , José Raúl Oubiña  7
Affiliations

Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis

Marcelo Rudzinski et al. Am J Trop Med Hyg. 2020 Dec.

Abstract

Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from Toxoplasma gondii lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain (P = 0.0015) or local TgCkAr-11-9 isolate (P = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients (P = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.

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Figures

Figure 1.
Figure 1.
Interferon gamma levels released from peripheral blood mononuclear cells (PBMC) obtained from control individuals (CI) and patients (P), under basal conditions (CI-PBMC and P-PBMC) and upon stimulation with TATL from either ME49 reference strain or TgCkAr-11-9 local isolate, as registered in CRA are shown. A significant increase in the release of IFN-γ was observed upon P-PBMC stimulation with TATL from either ME49 (*P = 0.0015) or TgCkAr-11-9 (**P = 0.0012). TATL from TgCkAr11-9 local isolate induced a higher P-PBMC release of IFN-γ levels (median = 26.03 pg/mL) than ME49 reference strain (median = 12.88) in CRA from all tested patients (***P = 0.02). CRA = cytokine release assay; IFN-γ = interferon gamma.
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