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. 2020 Sep 7:28:e20200259.
doi: 10.1590/1678-7757-2020-0259.

In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish

Fatih Tulumbaci  1 , Mustafa Gungormus  2   3
Affiliations

In vitro remineralization of primary teeth with a mineralization-promoting peptide containing dental varnish

Fatih Tulumbaci et al. J Appl Oral Sci. .

Abstract

Mineralization-promoting peptides are attractive candidates for new remineralization systems. In previous studies, peptides have been applied as aqueous solutions, which is not a clinically relevant form.

Objective: This study aims to investigate the efficiency of a mineralization-promoting peptide, applied in varnish, on remineralizing artificial caries on primary teeth.

Methodology: 55 primary molars were collected. Specimens were immersed in a demineralizing solution for 7 days and then, divided into 7 groups: Baseline: No-remineralization, Placebo: Blank colophony, F: Colophony 5% fluoride, P: Colophony 10% peptide, P+F: Colophony 5% fluoride and 10% peptide, Embrace: Embrace™ varnish, Durashield: Durashield™ varnish. A mixture of 35% w/v colophony varnishes were prepared in ethanol and applied accordingly. Specimens were immersed in a remineralization solution for 4 weeks and it was evaluated using PLM and SEM. Lesion depth reduction was examined by one-way ANOVA.

Results: There was no significant difference in mean lesion depths between baseline (147.04 ± 10.18 μm) and placebo groups (139.73 ± 14.92 μm), between F (120.95 ± 12.23 μm) and Durashield (113.47 ± 14.36 μm) groups and between P (81.79 ± 23.15 μm) and Embrace (90.26 ± 17.72 μm) groups. Lesion depth for the P+F group (66.95±10.59 μm) was significantly higher compared to all other groups. All groups contained samples with subsurface demineralized regions. Number of subsurface demineralized regions were higher in fluoride-containing groups.

Conclusions: We conclude that the mineralization-promoting peptide (MPP3) is effective in this in vitro study and the peptide shows benefits over fluoride as it yields less subsurface demineralized regions.

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Conflict of interest statement

Disclosure statement

The authors declare no conflict of interest

Figures

Figure 1
Figure 1. Outline of the study design
Figure 2
Figure 2. Mean lesion depths based on PLM images (Same letters indicate no statistically significant difference)
Figure 3
Figure 3. (a-f) Representative PLM images used for measuring the lesion depth decrease (g-i): Representative PLM images of subsurface demineralized regions
Figure 4
Figure 4. SEM images and the corresponding elemental mapping for calcium and phosphate showing subsurface demineralized regions in a) Durashield, b) F and c) P+F groups. (*: acid resistant film)
Figure 5
Figure 5. Number of specimens with subsurface demineralized regions in the groups
See this image and copyright information in PMC

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