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. 1988 Jul;170(7):3301-4.
doi: 10.1128/jb.170.7.3301-3304.1988.

Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments

P M Ahrweiler  1 , C Frieden
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Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments

P M Ahrweiler et al. J Bacteriol. 1988 Jul.

Abstract

A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.

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References

    1. Biochim Biophys Acta. 1967 Dec 19;149(2):489-95 - PubMed
    1. J Bacteriol. 1988 Jul;170(7):3040-5 - PubMed
    1. J Bacteriol. 1973 Apr;114(1):309-22 - PubMed
    1. Nucleic Acids Res. 1980 May 24;8(10):2255-74 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1858-61 - PubMed

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