Acetaminophen-induced renal toxicity: preventive effect of silver nanoparticles

Abstract

Present study was planned to investigate the ameliorative effect of silver nanoparticles (AgNPs) on acetaminophen-induced nephrotoxicity. Our results demonstrate that therapy of AgNPs at three different doses (50, 100 and 150 μg/kg once only) prevented the acetaminophen (2 g/kg once only) induced acute renal toxicity. AgNPs treated animals also show less intensity in the histological alterations in kidneys and corroborating the results of analysis of serum urea and creatinine. In addition, AgNPs therapy prevented the acetaminophen-induced oxidative stress, which was confirmed by the alleviated lipid peroxidation, enhanced renal reduced glutathione content and restored enzymatic activities of superoxide dismutase, catalase and adenosine triphosphatase in kidney. Thus, our results demonstrate a possible protective potential of AgNPs on renal toxicity induced by acetaminophen. This study will definitely lead to the development of therapeutic drug against nephrotoxicity, after further clinical and preclinical studies.

Keywords: GSH; acetaminophen; nephrotoxicity; oxidative stress; silver nanoparticles.

Graphical Abstract

Figure 1

Histopathology of Kidney (×400); Kidney of control rat (A), Kidney of rat intoxicated with APAP 2 g/kg (B), Kidney of rat treated with AgNPs 50 mg/kg after APAP intoxication (C), Kidney of rat treated with AgNPs 100 mg/kg after APAP intoxication (D), Kidney of rat treated with AgNPs 150 mg/kg after APAP intoxication (E), Kidney of rat treated with Silymaran 50 mg/kg after APAP intoxication (F).