Fructosyl-Valine Orally Administrated to Chickens is Absorbed from Gastrointestinal Tract

Abstract

Amadori products are non-enzymatically formed by binding carbonyl groups and amino groups. Glycated amino acids generated by reacting amino acid and glucose are also in a group of Amadori products of which the transport and metabolism have been investigated mainly in mammals but not in avians. In the present study, therefore, we examined whether dietary fructosyl-valine, which is one of the glycated amino acids, orally administrated to chickens can be incorporated into blood or not. Fructosyl-valine was orally administrated to the chicken and blood samples were collected at 0, 20, 40, 60, 120 and 180 min after administration. Plasma concentration of fructosyl-valine was measured by using LC/MS. The plasma concentration of fructosyl-valine was increased by passing time from 0 to 180 min after administration, and no change was observed in the control group. Conclusively, it was clarified that fructosyl-valine orally administrated to the chicken could be absorbed from gastrointestinal tract and incorporated into blood.

Keywords: Amadori product; fructosyl-valine; gastrointestinal tract; glycation; oral administration; plasma concentration.

Fig. 1.

Time course change in plasma fructosyl-valine concentration in young chickens orally administrated with fructosyl-valine. Fructosyl-valine dissolved in distilled water (300 µmol/kg) was orally administrated to the crop of young chickens. Control chickens were administrated distilled water alone. At 20, 40, 60, 120 and 180 min (60 and 180 min in control) after administration, blood samples were taken from heart and plasma fructosyl-valine concentration was measured by using LC/MS. ^{a, b} Means with different superscript letters are significantly different (P<0.05). Values were means±SE. n=5.