Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jul 30;64(s2):3142.
doi: 10.4081/ejh.2020.3142.

Hepatocyte growth factor intervention to reduce myocardial injury and improve cardiac function on diabetic myocardial infarction rats

Zaiyong Zhang  1 , Cheng Long  2 , Yufeng Guan  3 , Mingcai Song  4
Affiliations

Hepatocyte growth factor intervention to reduce myocardial injury and improve cardiac function on diabetic myocardial infarction rats

Zaiyong Zhang et al. Eur J Histochem. .

Abstract

Acute myocardial infarction (AMI) is recognized to be a severe threat to people's health conditions and life quality. The accumulation of hepatocyte growth factor (HGF) in ischemic myocardium has been observed in both processes of experimental ischemia and reperfusion (I/R) and permanent coronary artery occlusion. The aim of the study was to investigate the effect of HGF on myocardial cell apoptosis, ventricular remodeling and cardiac function after myocardial infarction (MI) in diabetic rats, and to explore whether the effect is mediated by HGF/c-Met signaling pathway. MI significantly increases LVWI and RVWI and myocardial apoptotic index, and up-regulates the expression of HGF and c-Met at mRNA and protein levels in MI control group. The LVWI and RVWI, and myocardial apoptosis were reduced by treatment with HGF, which also increased the myocardial cell viability and the expression of HGF and c-Met. In summary, HGF significantly attenuates myocardial apoptosis and improves cardiac function after AMI in diabetic rats by further enhancing the activation of HGF/c-Met pathway.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Images of tissues in each group after Hematoxylin & Eosin (HE) staining, 200 × and 400×. S group, sham operation group; MI group, diabetic myocardial infarction control group; HGF+MI group, diabetic myocardial infarction HGF intervention group.
Figure 2.
Figure 2.
Images of tissues in each group after TUNEL staining, 200 × and 400×. S group, sham operation group; MI group, diabetic myocardial infarction control group; HGF+MI group, diabetic myocardial infarction HGF intervention group.
Figure 3.
Figure 3.
Cell viability evaluated by MTT assay in the three groups. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group. *P<0.05 compared with sham group; #P<0.05 compared with MI group.
Figure 4.
Figure 4.
Apoptosis rate evaluated by the flow cytometry in the three groups. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group.
Figure 5.
Figure 5.
HGF and c-Met mRNA expression in myocardial tissues. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group. *P<0.05 compared with sham group; #P<0.05 compared with MI group.
Figure 6.
Figure 6.
HGF and c-Met protein expression in myocardial tissues. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group. *P<0.05 compared with sham group; #P<0.05 compared with MI group.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Aniwan S, Pardi DS, Tremaine WJ, Loftus EV. Increased risk of acute myocardial infarction and heart failure in patients with inflammatory bowel diseases. Clin Gastroenterol Hepatol 2018;16:1607-15. - PMC - PubMed
    1. Echouffo-Tcheugui JB, Kolte D, Khera S, Aronow HD, Abbott JD, Bhatt DL, et al. Diabetes mellitus and cardiogenic shock complicating acute myocardial infarction. Am J Med 2018;131:778-86. - PubMed
    1. Sardella G, Mancone M, Stio RE, Cavallo E, Di Roma A, Colantonio R, et al. Prasugrel or ticagrelor in ST-segment-elevation myocardial infarction patients with diabetes mellitus. Circulation 2017;136:602-4. - PubMed
    1. Nakamura T, Mizuno S. The discovery of hepatocyte growth factor (HGF) and its significance for cell biology, life sciences and clinical medicine. Proc Jpn Acad Ser B Phys Biol Sci 2010;86:588-610. - PMC - PubMed
    1. Otsuki Y, Nakamura Y, Harada S, Yamamoto Y, Ogino K, Morikawa K, et al. Adipose stem cell sheets improved cardiac function in the rat myocardial infarction, but did not alter cardiac contractile responses to beta-adrenergic stimulation. Biomed Res 2015;36:11-9. - PubMed

MeSH terms

Substances