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. 2020 Jul 30;64(s2):3142.
doi: 10.4081/ejh.2020.3142.

Hepatocyte growth factor intervention to reduce myocardial injury and improve cardiac function on diabetic myocardial infarction rats

Zaiyong Zhang  1 , Cheng Long  2 , Yufeng Guan  3 , Mingcai Song  4
Affiliations

Hepatocyte growth factor intervention to reduce myocardial injury and improve cardiac function on diabetic myocardial infarction rats

Zaiyong Zhang et al. Eur J Histochem. .

Abstract

Acute myocardial infarction (AMI) is recognized to be a severe threat to people's health conditions and life quality. The accumulation of hepatocyte growth factor (HGF) in ischemic myocardium has been observed in both processes of experimental ischemia and reperfusion (I/R) and permanent coronary artery occlusion. The aim of the study was to investigate the effect of HGF on myocardial cell apoptosis, ventricular remodeling and cardiac function after myocardial infarction (MI) in diabetic rats, and to explore whether the effect is mediated by HGF/c-Met signaling pathway. MI significantly increases LVWI and RVWI and myocardial apoptotic index, and up-regulates the expression of HGF and c-Met at mRNA and protein levels in MI control group. The LVWI and RVWI, and myocardial apoptosis were reduced by treatment with HGF, which also increased the myocardial cell viability and the expression of HGF and c-Met. In summary, HGF significantly attenuates myocardial apoptosis and improves cardiac function after AMI in diabetic rats by further enhancing the activation of HGF/c-Met pathway.

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Figures

Figure 1.
Figure 1.
Images of tissues in each group after Hematoxylin & Eosin (HE) staining, 200 × and 400×. S group, sham operation group; MI group, diabetic myocardial infarction control group; HGF+MI group, diabetic myocardial infarction HGF intervention group.
Figure 2.
Figure 2.
Images of tissues in each group after TUNEL staining, 200 × and 400×. S group, sham operation group; MI group, diabetic myocardial infarction control group; HGF+MI group, diabetic myocardial infarction HGF intervention group.
Figure 3.
Figure 3.
Cell viability evaluated by MTT assay in the three groups. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group. *P<0.05 compared with sham group; #P<0.05 compared with MI group.
Figure 4.
Figure 4.
Apoptosis rate evaluated by the flow cytometry in the three groups. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group.
Figure 5.
Figure 5.
HGF and c-Met mRNA expression in myocardial tissues. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group. *P<0.05 compared with sham group; #P<0.05 compared with MI group.
Figure 6.
Figure 6.
HGF and c-Met protein expression in myocardial tissues. S, sham operation group; MI, diabetic myocardial infarction control group; HGF+MI, diabetic myocardial infarction HGF intervention group. *P<0.05 compared with sham group; #P<0.05 compared with MI group.
See this image and copyright information in PMC

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