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. 2020 Sep;48(9):300060520931245.
doi: 10.1177/0300060520931245.

Effect of parathyroid hormone-related protein on intracellular calcium ion and cyclic adenosine monophosphate concentrations in cardiac fibroblasts

Ping Zhou  1 , Qiong Xiao  2 , Zhao-Ting Su  3 , Lin Zhu  1 , Fang-Xia Jin  4 , Xuan-Yi Du  3
Affiliations

Effect of parathyroid hormone-related protein on intracellular calcium ion and cyclic adenosine monophosphate concentrations in cardiac fibroblasts

Ping Zhou et al. J Int Med Res. 2020 Sep.

Abstract

Objective: This study aimed to determine the effect of parathyroid hormone-related protein (PTHrP) on proliferation of cardiac fibroblasts (CFs) in primary cultures of neonatal Wistar rats.

Methods: Different PTHrP concentrations were added to CFs of neonatal Wistar rats and the cells were grouped according to the concentrations added. A verapamil (VPL) group and a calcitriol (CAL) group were also established. Changes in cell proliferation and in cyclic adenosine monophosphate and calcium ion levels were identified and recorded.

Results: We found that as the concentration of PTHrP increased, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT, a tetrazolium salt) colorimetric absorbance values (A values) decreased. These values in the PTHrP groups were significantly lower than those in the control group. MTT colorimetric A values and 3H-thymidine deoxyribose intake were lower in the VPL group, low-dose CAL group, and the PTHrP 10-7 mol/L group compared with the control group. However, MTT colorimetric A values and 3H-thymidine deoxyribose intake were higher in the high-dose CAL group than in the PTHrP 10-7 mol/L group. As PTHrP concentrations increased, intracellular cyclic adenosine monophosphate concentrations also increased.

Conclusion: PTHrp, VPL, and low-dose CAL inhibit proliferation of CFs, while high-dose CAL promotes proliferation of CFs.

Keywords: Parathyroid hormone-related protein; calcitriol; calcium ion; cardiac muscle; cyclic adenosine monophosphate (cAMP); fibroblasts; verapamil.

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Figures

Figure 1.
Figure 1.
Effects of different concentrations of PTHrP on cAMP levels in rat cardiac fibroblasts PTHrP, parathyroid hormone-related protein; cAMP, cyclic adenosine monophosphate; ver, verapamil; cal, calcitriol. *Compared with the control group, P < 0.01; #compared with the PTHrp+Cal−9 group, P < 0.05; ¥ compared with the control group, P > 0.05.
Figure 2.
Figure 2.
Effects of different concentrations of PTHrP on calcium ion concentrations in rat cardiac fibroblasts. PTHrP, parathyroid hormone-related protein; ver, verapamil; cal, calcitriol. *P < 0.05.
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