Serum microRNA is a biomarker for post-operative monitoring in glioma

Andrew Morokoff^{1

2

3}, Jordan Jones^{4

5}, Hong Nguyen⁴, Chenkai Ma⁴, Arian Lasocki⁶, Frank Gaillard⁶, Iwan Bennett⁴, Rod Luwor⁴, Stanley Stylli⁵, Lucia Paradiso⁴, Rachel Koldej⁷, Iddo Paldor⁵, Ramyar Molania⁸, Terence P Speed⁸, Andrew Webb⁸, Guiseppe Infusini⁸, Jason Li⁹, Charles Malpas¹⁰, Tomas Kalincik¹⁰, Katharine Drummond^{4

5}, Tali Siegal¹¹, Andrew H Kaye^{4

12}

Affiliations

¹ Department of Surgery, University of Melbourne, Parkville, VIC, Australia. morokoff@unimelb.edu.au.
² Department of Neurosurgery, Royal Melbourne Hospital, Parkville, VIC, Australia. morokoff@unimelb.edu.au.
³ Department of Surgery, University of Melbourne, Royal Melbourne Hospital Parkville, VIC, 3050, Australia. morokoff@unimelb.edu.au.
⁴ Department of Surgery, University of Melbourne, Parkville, VIC, Australia.
⁵ Department of Neurosurgery, Royal Melbourne Hospital, Parkville, VIC, Australia.
⁶ Department of Radiology, University of Melbourne, Parkville, VIC, Australia.
⁷ Department of Medicine, University of Melbourne, Parkville, VIC, Australia.
⁸ Walter and Eliza Hall Institute, Parkville, VIC, Australia.
⁹ Peter MacCallum Cancer Centre, Parkville, VIC, Australia.
¹⁰ Clinical Outcomes Research Unit, Royal Melbourne Hospital, Parkville, VIC, Australia.
¹¹ Neuro-Oncology, Davidoff Institute of Oncology, Rabin Medical Center, Petach Tikva, Israel.
¹² Department of Neurosurgery, Hadassah Hebrew University, Jerusalem, Israel.

PMID: 32915353
DOI: 10.1007/s11060-020-03566-w

Serum microRNA is a biomarker for post-operative monitoring in glioma

Andrew Morokoff et al. J Neurooncol. 2020 Sep.

. 2020 Sep;149(3):391-400.

doi: 10.1007/s11060-020-03566-w. Epub 2020 Sep 11.

Authors

Affiliations

¹ Department of Surgery, University of Melbourne, Parkville, VIC, Australia. morokoff@unimelb.edu.au.
² Department of Neurosurgery, Royal Melbourne Hospital, Parkville, VIC, Australia. morokoff@unimelb.edu.au.
³ Department of Surgery, University of Melbourne, Royal Melbourne Hospital Parkville, VIC, 3050, Australia. morokoff@unimelb.edu.au.
⁴ Department of Surgery, University of Melbourne, Parkville, VIC, Australia.
⁵ Department of Neurosurgery, Royal Melbourne Hospital, Parkville, VIC, Australia.
⁶ Department of Radiology, University of Melbourne, Parkville, VIC, Australia.
⁷ Department of Medicine, University of Melbourne, Parkville, VIC, Australia.
⁸ Walter and Eliza Hall Institute, Parkville, VIC, Australia.
⁹ Peter MacCallum Cancer Centre, Parkville, VIC, Australia.
¹⁰ Clinical Outcomes Research Unit, Royal Melbourne Hospital, Parkville, VIC, Australia.
¹¹ Neuro-Oncology, Davidoff Institute of Oncology, Rabin Medical Center, Petach Tikva, Israel.
¹² Department of Neurosurgery, Hadassah Hebrew University, Jerusalem, Israel.

PMID: 32915353
DOI: 10.1007/s11060-020-03566-w

Erratum in

Correction to: Serum microRNA is a biomarker for post-operative monitoring in glioma.
Morokoff A, Jones J, Nguyen H, Ma C, Lasocki A, Gaillard F, Bennett I, Luwor R, Stylli S, Paradiso L, Koldej R, Paldor I, Molania R, Speed TP, Webb A, Infusini G, Li J, Malpas C, Kalincik T, Drummond K, Siegal T, Kaye AH. Morokoff A, et al. J Neurooncol. 2020 Sep;149(3):401. doi: 10.1007/s11060-020-03630-5. J Neurooncol. 2020. PMID: 33026635

Abstract

Purpose: A circulating biomarker has potential to provide more accurate information for glioma progression post treatment, however no such biomarker is currently available. We aimed to discover a microRNA serum biomarker for longitudinal monitoring of glioma patients.

Methods: A prospectively collected cohort of 91 glioma patients and 17 healthy controls underwent pre and post-operative serum miRNA profiling using Nanostring®. Differentially expressed miRNAs were discovered using a machine learning random forest analysis. Candidate miRNAs were then assessed by droplet digital PCR in 11 patients with multiple follow up samples and compared to tumor volume based on magnetic resonance imaging.

Results: A 9-gene miRNA signature was identified that could distinguish between glioma and healthy controls with 99.8% accuracy. Two miRNAs miR-223 and miR-320e, best demonstrated dynamic changes that correlated closely with tumor volume in LGG and GBM respectively. Importantly, miRNA levels did not increase in two cases of pseudo-progression, indicating the potential utility of this test in guiding treatment decisions.

Conclusions: We identified a highly accurate 9-miRNA signature associated with glioma serum. Additionally, we observed dynamic changes in specific miRNAs correlating with tumor volume over long-term follow up. These results support a large prospective validation study of serum miRNA biomarkers in glioma.

Keywords: Biomarkers; Glioblastoma; Glioma; Liquid biopsy; miRNA.

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References

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Serum microRNA is a biomarker for post-operative monitoring in glioma

Affiliations

Serum microRNA is a biomarker for post-operative monitoring in glioma

Authors

Affiliations

Erratum in

Abstract

References

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LinkOut - more resources

Full Text Sources

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Medical