Abiotic stress elements in in vitro potato (Solanum tuberosum L.) exposed to air-based and liquid-based ultrasound: A comparative transcriptomic assessment

Abstract

Ultrasound (US) can modify the plant growth and development. Previous assessments of the transcriptome of in vitro potato (Solanum tuberosum L.) exposed to US transmitted through air (AB-US) or liquid (PE-US) revealed the up- or down-regulation of several stress-related differentially expressed genes (DEGs) related to abiotic stress. In a bid to better characterize stress-related elements over a four-week period, the transcriptome of AB-US was compared to that of PE-US. When comparing the controls of both treatments, DEGs related to hypoxia were not detected. Nevertheless, hypoxia-related DEGs were detected in the combination of liquid medium and ultrasonication. DEGs coding for chitinase, peroxidase, glutathione-S-transferase, transcription factors of ERF (ethylene responsive factor), DREB (dehydration-responsive element-binding), WRKY and MYB were also significantly highly expressed in PE-US, relative to AB-US. Up- and down-regulation of DEGs related to metabolic processes, and enzymes of the antioxidant system also confirm that PE-US is a more acute abiotic stress than AB-US. KEY MESSAGE: A transcriptomic analysis revealed that liquid-based ultrasonication was a stronger abiotic stressor than air-based ultrasonication. Of particular interest were the heat shock proteins and transcription factors in this comparison. Despite the ultrasound stress, explants survived and plantlets developed.

Keywords: Abiotic stress; Differentially expressed genes; Heat shock proteins; Plant growth.