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. 2020 Nov;20(6):e209-e211.
doi: 10.7861/clinmed.2020-0555. Epub 2020 Sep 11.

The clinical sensitivity of a single SARS-CoV-2 upper respiratory tract RT-PCR test for diagnosing COVID-19 using convalescent antibody as a comparator

Abigail Holborow  1 , Hibo Asad  1 , Lavinia Porter  2 , Poppy Tidswell  2 , Claire Johnston  1 , Ian Blyth  1 , Alice Bone  1 , Brendan Healy  3
Affiliations

The clinical sensitivity of a single SARS-CoV-2 upper respiratory tract RT-PCR test for diagnosing COVID-19 using convalescent antibody as a comparator

Abigail Holborow et al. Clin Med (Lond). 2020 Nov.

Abstract

The clinical false negative rate of reverse transcriptase polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 on a single upper respiratory tract sample was calculated using convalescent antibody testing as a comparator. The sensitivity in symptomatic individuals was 86.2% (25/29). Of the missed cases, one (3.5%) was detected by repeat RT-PCR, one by CT thorax and two (7.1%) by convalescent antibody. The clinical false negative rate of a single RT-PCR on an upper respiratory tract sample of 14% in symptomatic patients is reassuring when compared to early reports. This report supports a strategy of combining repeat swabbing, use of acute and convalescent antibody testing and CT thorax for COVID-19 diagnosis.

Keywords: COVID-19; COVID-19 antibody; SARS-CoV-2 RT-PCR; clinical sensitivity.

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