Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Mar 15;250(3):925-8.
doi: 10.1042/bj2500925.

Interaction of the trp repressor from Escherichia coli with a constitutive trp operator

L R Chandler  1 , A N Lane
Affiliations

Interaction of the trp repressor from Escherichia coli with a constitutive trp operator

L R Chandler et al. Biochem J. .

Abstract

The interaction of the trp repressor from Escherichia coli with a 20 bp fragment of DNA (CGTACTGATT.AATCAGTACG) corresponding to a mutant trp operator was studied by c.d. in the presence and absence of the co-repressor, L-tryptophan, and as a function of the concentration of K+ and Na+ ions. The affinity of the repressor for the mutant operator in the presence of tryptophan is about three orders of magnitude lower than the wild-type sequence. Binding in the absence of tryptophan is about 100-fold weaker than to the wild-type. The dependence of the dissociation constant on the concentration of K+ or Na+ is weak [d(log Ks)/d(log[M+]) = 2.5], and independent of the cation, indicating that electrostatic interactions are not as important for this repressor as for others.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4808-12 - PubMed
    1. J Biomol Struct Dyn. 1985 Dec;3(3):529-36 - PubMed
    1. J Mol Biol. 1978 May 15;121(2):179-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 May;79(10):3120-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Feb;80(3):668-72 - PubMed

Publication types